FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968142
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACATACTACGCAGACTCTGTGAAGGGCCGATTCACCATGTCCAGAGA39690.4099605223200729No Hit
GTGTATTACTGTGCGAGAGAGGTTCACTACAACGACTACGGTATGGATGT25140.25967265132594186No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA22210.22940849586114434No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18870.19490942444393486No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18030.18623301127313968No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16950.17507762291068873No Hit
GTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA15160.15658859960625612No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14740.15225039302085852No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14630.1511141960580163No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13790.1424377828872211No Hit
GTATGTGGTAGTCCTACTAATACTAATGTATGAAACCCACTCCAGCCCCT13630.14078513275945057No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13630.14078513275945057No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13360.13799628566883784No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13060.13489756667926814No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12070.12467179401368807No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11590.11971384363037654No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11540.11919739046544825No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11470.11847435603454864No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.11733815907170643No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC11030.11392956818317974No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATGTCCAGAGACAACG10730.11083084919361003No Hit
GTATTACTGTGCGAGAGAGGTTCACTACAACGACTACGGTATGGATGTCT10670.11021110539569609No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10300.10638935197522677No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9710.100295204629073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)