FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968142
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCACATACTACGCAGACTCTGTGAAGGGCCGATTCACCATGTCCAGAGA32610.33683075416622765No Hit
GTGTATTACTGTGCGAGAGAGGTTCACTACAACGACTACGGTATGGATGT24180.24975675055931879No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA21540.22248802345110533No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20060.20720100976922806No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15450.15958402796284016No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15440.15948073732985452No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14530.1500812897281597No Hit
GTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA14370.1484286396003892No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14200.14667269883963302No Hit
GTATGTGGTAGTCCTACTAATACTAATGTATGAAACCCACTCCAGCCCCT13630.14078513275945057No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13320.13758312313689522No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12240.12642773477444424No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12180.1258079909765303No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11860.1225026907209893No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11790.12177965629008969No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11310.11682170590677814No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10430.10773213020404031No Hit
GTATTACTGTGCGAGAGAGGTTCACTACAACGACTACGGTATGGATGTCT10080.10411695804954232No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9990.10318734235267141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9700.068.255481
TCAACGC9600.065.160974
ATCAACG9900.063.906073
CAACGCA10300.060.745125
AACGCAG11050.056.6280026
TATCAAC11400.056.128032
CGCAGAG13200.046.8620538
GGTATCA5000.046.057611
CCGTTGA3650.041.3831189
GTACATG20600.037.7292331
CCCCGTT3900.036.884147
GTCATAG10350.036.851651
GCAGAGT16700.036.6099559
TCATAGG10350.036.1208232
GAATACG2002.4374458E-1035.9638989
TACATGG21250.035.1859322
ACATGGG21500.034.776793
CAGCGCT5200.034.580679
ATAGGTG10250.034.372554
CCCGTTG4400.034.3291748
Produced by FastQC (version 0.11.5)