FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490286
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGAGTAGGTCTTCT76310.5120493650212107No Hit
GTAGTACATAGCCGTGTCAGCGGCGGTCACAGAGCTCACCGTCAGGGAGA70810.47514369725005806No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32540.21834735077696496No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30600.20532971523586746No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29430.19747887318273136No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27930.18741369106332612No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27260.1829179097166584No Hit
ACCTACTACAGCCCGTCCCTCAGGGGTCGAGTCACCATAGTGGAAGACAC27050.18150878421994168No Hit
GCTATGTACTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGAGTAGGTCT26520.17795241987108515No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25890.17372504338093495No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25550.1714436021005364No Hit
ACTACAGCCCGTCCCTCAGGGGTCGAGTCACCATAGTGGAAGACACGTCC25360.1701686790320784No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24540.16466637947347018No Hit
GTACTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGAGTAGGTCTTCTTT24410.1637940636897884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23160.15540641192361734No Hit
GTACATAGCCGTGTCAGCGGCGGTCACAGAGCTCACCGTCAGGGAGAACT23080.15486960221058238No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22850.15332627428560694No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22620.15178294636063144No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20810.13963762660321577No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20750.13923501931843954No Hit
GTAGTAGGTGAACCCATTATAAGAAATACTCCCTATCCACTCCAGCCCCA20450.1372219828945585No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18140.12172160243067438No Hit
GGATAGGGAGTATTTCTTATAATGGGTTCACCTACTACAGCCCGTCCCTC17970.12058088179047512No Hit
CAGTAGTACATAGCCGTGTCAGCGGCGGTCACAGAGCTCACCGTCAGGGA17880.1199769708633108No Hit
GTCTCTGGTGACTCCGTCAATAGTCCTAGTTACTATTGGGTCTGGATCCG17730.11897045265137028No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG17240.11568249315903122No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT16760.11246163488082153No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16750.11239453366669216No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16670.11185772395365721No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC16590.11132091424062227No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA16470.11051569967106985No Hit
GTCTCCAGCCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCT16000.10736194260698953No Hit
CTCCAGGGATGGGGCTGGAGTGGATAGGGAGTATTTCTTATAATGGGTTC15910.10675803167982521No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15820.1061541207526609No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15770.10581861468201405No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15510.10407398311465049No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15490.10393978068639173No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14990.10058471997992331No Hit
TCCCAGATGCGTCCTGTCCCTCCTGCAACTGCGGGAGTCGGGCCCAGGAC14920.10011501148101773No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)