FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490286
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGAGTAGGTCTTCT70170.47084921954577846No Hit
GTAGTACATAGCCGTGTCAGCGGCGGTCACAGAGCTCACCGTCAGGGAGA65570.439982661046269No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30470.2044573994521857No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30220.20277986909895146No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25470.17090679238750145No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24310.1631230515484947No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA24160.16211653333655418No Hit
ACCTACTACAGCCCGTCCCTCAGGGGTCGAGTCACCATAGTGGAAGACAC24060.1614455211952605No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24060.1614455211952605No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC22860.1533933754997363No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22810.15305786942908944No Hit
GCTATGTACTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGAGTAGGTCT22300.14963570750849167No Hit
GTACATAGCCGTGTCAGCGGCGGTCACAGAGCTCACCGTCAGGGAGAACT22170.14876339172480985No Hit
GTACTACTGTGCGAGTTCGTCAGTGGCTGGCACCAGAGTAGGTCTTCTTT21760.14601224194550577No Hit
ACTACAGCCCGTCCCTCAGGGGTCGAGTCACCATAGTGGAAGACACGTCC21640.14520702737595334No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21470.14406630673575407No Hit
GTAGTAGGTGAACCCATTATAAGAAATACTCCCTATCCACTCCAGCCCCA19980.13406822583047817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.13346431490331387No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18320.12292942428500302No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18300.12279522185674428No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17970.12058088179047512No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17430.11695741622748922No Hit
GTCTCTGGTGACTCCGTCAATAGTCCTAGTTACTATTGGGTCTGGATCCG17120.1148772785894788No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17030.11427336766231448No Hit
CAGTAGTACATAGCCGTGTCAGCGGCGGTCACAGAGCTCACCGTCAGGGA16940.11366945673515017No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16920.11353525430689143No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16340.10964338388738806No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16330.10957628267325868No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT16280.10924077660261185No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16210.10877106810370626No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15280.102530655189675No Hit
GGATAGGGAGTATTTCTTATAATGGGTTCACCTACTACAGCCCGTCCCTC14940.10024921390927646No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26900.066.640471
ATCAACG32350.053.6440053
TCAACGC32650.053.1511044
TATCAAC34300.050.804212
CAACGCA34000.050.1935465
GGTATCA13600.049.230591
AACGCAG34800.049.0413176
CGCAGAG38700.043.727028
ATAGCCG33150.037.362678
GCAGAGT45800.036.7837459
TAGCCGT33800.036.636789
ACGCAGA47500.035.777627
TACATAG36150.034.460085
GTACATG40300.034.120811
TAGTACA37550.033.3670462
TACATGG41100.032.587392
GTAGTAC38550.032.494951
ACATAGC38000.032.4045146
GTACATA38800.032.2920764
CATAGCC39000.031.5736317
Produced by FastQC (version 0.11.5)