FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732075
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36590.21124951286751442No Hit
GTCTACATGGAGCTGAGCAGTCTGAGGTCTGAAGACACGGCCGTGTTTTA35350.20409046952354834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26630.15374622923372258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26060.15045537866431882No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAAGTCTCCTGCA21810.1259183349450803No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19950.11517976992913126No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19370.11183118513921164No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAA19080.11015689274425185No Hit
GACTTACAGTACCCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC18720.10807846080568104No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18300.10565362354401511No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17940.10357519160544433No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAATCT17640.10184316498996868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32950.072.3301541
TCAACGC37950.061.0964474
ATCAACG39000.059.6361733
TATCAAC41450.056.2849542
CAACGCA41300.055.966335
AACGCAG42950.054.151596
CGCAGAG49500.047.1315428
ACGCAGA53250.043.812427
GCAGAGT69800.033.830049
TACGCAA1402.8790626E-530.859947
GTCTACA23650.030.4449751
GTACATG44550.029.2534851
GTACACG8700.028.9681435
GGTATCA36350.027.929391
ACATGGG46000.027.5503233
TACACGG9300.027.0992326
CAGAGTA59950.026.2989869
CTACATG23800.026.0191633
TCTACAT25250.025.3805182
GAATACG3001.8415449E-823.9973249
Produced by FastQC (version 0.11.5)