FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602418
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCGCGGCGGTCATAGAGCTCAGCCTCAGGGAGA33180.5507803551686703No Hit
GTATATTACTGTGCGAGAGCCTCCCATTGTACTGGTACCAGCTGCTACCA25220.4186461891908941No Hit
CCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG16960.2815320923345584No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16750.2780461407195668No Hit
GTCTCTGGTGGGTCCTTCAGTGATTACTACTGGAGCTGGATCCGCCAGCC13500.22409688953517326No Hit
ATATACAGCCGTGTCCGCGGCGGTCATAGAGCTCAGCCTCAGGGAGAACT12910.21430302547400643No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCGATGATCACTA12890.21397103008210247No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC11340.18824138720954553No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10700.1776175346686188No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10240.1699816406548277No Hit
GCTGTATATTACTGTGCGAGAGCCTCCCATTGTACTGGTACCAGCTGCTA10190.1691516521750678No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG10090.167491675215548No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9870.16383972590460444No Hit
CTATTGGTCATCTATTTTGATAGTGACCGGCCCTCAGGGATCCCTGAGCG9680.16068576968151682No Hit
GTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAG9650.16018777659366087No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9610.15952378580985296No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCG9410.15620383189081335No Hit
GTAGTAGTGATCATCGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTA9250.15354786875558168No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9190.1525518825798698No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA9190.1525518825798698No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT9010.14956392405273417No Hit
TAGTAGTAGTGATCATCGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCC8970.14889993326892623No Hit
ATATTACTGTGCGAGAGCCTCCCATTGTACTGGTACCAGCTGCTACCAAA8730.1449159885660787No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8410.13960406229561534No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC8370.13894007151180743No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8290.13761208994419158No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC8140.13512212450491187No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8090.13429213602515197No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8000.13279815676158416No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7940.13180217058587226No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7910.13130417749801632No Hit
CAGTAATATACAGCCGTGTCCGCGGCGGTCATAGAGCTCAGCCTCAGGGA7730.1283162189708807No Hit
GTACAATGGGAGGCTCTCGCACAGTAATATACAGCCGTGTCCGCGGCGGT7440.12350228578827327No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7400.12283829500446533No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7130.11835635721376186No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6830.11337642633520247No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG6690.11105245859187474No Hit
ATTTTGATAGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCC6640.11022247011211485No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCAC6540.10856249315259504No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6460.1072345115849792No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6430.10673651849712326No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG6430.10673651849712326No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6430.10673651849712326No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6380.10590653001736336No Hit
GGATAGTAGTAGTGATCATCGGGTGTTCGGCGGAGGGACCAAGTTGACCG6380.10590653001736336No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6360.1055745346254594No Hit
GACCAATAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTT6310.10474454614569949No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATTCTGGGAACACGG6210.10308456918617968No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6150.10208858301046782No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6150.10208858301046782No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6110.10142459222665991No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT6060.10059460374689999No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGGGTCCTTCAGTGATTACTACTGGA6050.10042860605094801No Hit
CTATCAAAATAGATGACCAATAGAGGGGCCTGGCCTGGCTTCTGCTGGTA6030.10009661065904406No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)