FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137724
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATAATCAGCCTCATCGCCAGACTGGAGCCCACTGATGGTCAGGGAGG49600.435958105832346No Hit
ATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG44580.3918349265727013No Hit
GATTATCACTGTGCAGCATGGGATGACAGCCTGTATGGTGTGGTCTTCGG40510.3560617513562164No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC39460.3468327995190398No Hit
GATTATTATTATACATGAGGAGTTTGGGGGCCGTTCCCGGGAGTTGCTGG38180.3355822677556244No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC33070.29066803548136455No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCACACCATACAGG30540.2684306562927388No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCT30250.26588170769009006No Hit
CCATACAGGCTGTCATCCCATGCTGCACAGTGATAATCAGCCTCATCGCC28070.24672064578052322No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAATTCAGCTTCAGGGAGA27040.23766748350214992No Hit
GTCTATAACAGCGGGAACGCCGACTACAACCCCTCCTTCAAGAGTCGAGT26780.23538221923770616No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT26620.23397590276727923No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCAC24120.2120022079168586No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC23970.2106837862258333No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGTCAGAGGGTCAC23590.20734378460856936No Hit
GTATAATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT22770.20013641269763144No Hit
GTCTCTGGTGGCTCCATCAATACTTACCACTGGAGTTGGATCCGGCAGTC22610.1987300962272045No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22610.1987300962272045No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22020.1935443042425052No Hit
TTATTATACATGAGGAGTTTGGGGGCCGTTCCCGGGAGTTGCTGGTACCA21450.1885343018166093No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21050.18501851064054198No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20730.18220587769968816No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20470.1799206134352444No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20200.177547454391399No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20140.1770200857149889No Hit
GTTATAGACATAGCCAATCCACTCCAGTCCCTTCCCTGGGGACTGCCGGA19570.17201008328909295No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA18840.16559376439277013No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18340.161199025422686No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18050.1586500768200372No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17960.15785902380542205No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17920.15750744468781533No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC17890.15724376034961027No Hit
GTATGGTGTGGTCTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGC17880.1571558655702086No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17530.1540795482911497No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17510.15390375873234632No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA17410.1530248109383295No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17400.15293691615892782No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCACACCATACAGGC17320.15223375792371435No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17280.15188217880610763No Hit
GTATTACTTCCGATGTTGGAGGTGCCCCCAGAACAAGAGATGGTGACCCT17220.15135481012969754No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGTCGTGACAGTGGCCTGGA16800.14766322939482684No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT16660.1464327024832033No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT16640.14625691292439993No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16380.1439716486599562No Hit
TCTCATAAGTGACTTCTACCCGGGAGTCGTGACAGTGGCCTGGAAGGCAG16380.1439716486599562No Hit
GTGCAGCATGGGATGACAGCCTGTATGGTGTGGTCTTCGGCGGGGGGACC16010.14071954182209395No Hit
ATGCTGCACAGTGATAATCAGCCTCATCGCCAGACTGGAGCCCACTGATG15910.1398405940280771No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14710.12929322049987518No Hit
GTTTATTACTGTGCGAGACGACATAGTGGGAACTTCTACGGCTGGTTCGA14630.12859006226466171No Hit
ATCCCATGCTGCACAGTGATAATCAGCCTCATCGCCAGACTGGAGCCCAC14600.12832637792645668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.12788690402944825No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14420.1267442718972264No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT14220.12498637630919275No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG14040.12340427027996245No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13960.122701112044749No Hit
TTCCAGGCCACTGTCACGACTCCCGGGTAGAAGTCACTTATGAGACACAC13850.12173426947133048No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13830.12155847991252712No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13780.1211190060155187No Hit
CTCTTGTTCTGGGGGCACCTCCAACATCGGAAGTAATACTGTAAACTGGT13050.11470268711919586No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTG12970.1139995288839824No Hit
GCATGGGATGACAGCCTGTATGGTGTGGTCTTCGGCGGGGGGACCAAGCT12510.10995636903150502No Hit
CCTCATGTATAATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATTCT12330.10837426300227472No Hit
TAATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA12310.10819847344347136No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12260.10775899954646295No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12070.10608899873783097No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12040.10582531439962592No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCC12020.10564952484082256No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGTCGTGACAGTGGC11950.10503426138501078No Hit
CCTGTATGGTGTGGTCTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTC11910.10468268226740404No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCCGCC11840.10406741881159227No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG11770.10345215535578049No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11700.10283689189996871No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11520.10125478587073843No Hit
ACTGTAAACTGGTACCAGCAACTCCCGGGAACGGCCCCCAAACTCCTCAT11520.10125478587073843No Hit
CCCCTGAGGGCCGCAGATTATTATTATACATGAGGAGTTTGGGGGCCGTT11410.10028794329731991No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG11380.10002425895911486No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.084.747861
GTATCAA21450.067.837811
TCAACGC21300.066.924494
ATCAACG21350.066.773633
CAACGCA21600.065.994985
TATCAAC22550.063.539562
AACGCAG22700.062.7969936
GAATACG3600.059.9928059
CGCAGAG25150.056.67718
GCAGAGT30150.047.516699
GTGCTAG5050.045.6463741
ACGCAGA33100.043.0643237
CAGCGCT5750.038.8127379
TACATGG24400.036.2955132
AGAATAC6150.036.288338
GTACATG24250.036.240631
CTAGAGA6450.035.7182164
ACATGGG25200.034.571843
GCAGCGC6700.034.3839348
GCTAGAG6300.034.286123
Produced by FastQC (version 0.11.5)