FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137724
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG51020.4484391645073849No Hit
GATTATCACTGTGCAGCATGGGATGACAGCCTGTATGGTGTGGTCTTCGG40810.35869859473826693No Hit
GATTATTATTATACATGAGGAGTTTGGGGGCCGTTCCCGGGAGTTGCTGG40090.3523701706213458No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC39780.3496454324598936No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC36730.3228375247423804No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCT34660.3046433054062321No Hit
GTGATAATCAGCCTCATCGCCAGACTGGAGCCCACTGATGGTCAGGGAGG31090.27326486915983134No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCACACCATACAGG27780.24417169717787443No Hit
GTCTATAACAGCGGGAACGCCGACTACAACCCCTCCTTCAAGAGTCGAGT27510.24179853813402896No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAATTCAGCTTCAGGGAGA26420.23221800717924557No Hit
GTATAATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT25960.22817484732676815No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC25480.2239558979154874No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGTCAGAGGGTCAC25110.22070379107762517No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT25050.22017642240121504No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24390.214375366960704No Hit
TTATTATACATGAGGAGTTTGGGGGCCGTTCCCGGGAGTTGCTGGTACCA23590.20734378460856936No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23100.20303694041788695No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCAC22940.20163062394746No Hit
GTCTCTGGTGGCTCCATCAATACTTACCACTGGAGTTGGATCCGGCAGTC22190.19503851549233384No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20810.1829090359349016No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC20680.18176640380267972No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20230.17781113872960402No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19980.17561376924456196No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19980.17561376924456196No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC19590.17218587284789633No Hit
GTTATAGACATAGCCAATCCACTCCAGTCCCTTCCCTGGGGACTGCCGGA19410.17060376681866604No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18620.1636600792459331No Hit
GTATGGTGTGGTCTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTCAGC18610.1635721844665314No Hit
CCATACAGGCTGTCATCCCATGCTGCACAGTGATAATCAGCCTCATCGCC18200.15996849851106246No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA17400.15293691615892782No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17400.15293691615892782No Hit
GTATTACTTCCGATGTTGGAGGTGCCCCCAGAACAAGAGATGGTGACCCT17250.15161849446790257No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17210.15126691535029585No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17130.15056375711508238No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16960.14906954586525378No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16900.1485421771888437No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16770.1473995450566218No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16600.1459053338067932No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16480.144850596453973No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGTCGTGACAGTGGCCTGGA16360.14379585910115283No Hit
TCTCATAAGTGACTTCTACCCGGGAGTCGTGACAGTGGCCTGGAAGGCAG16140.14186217395431583No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16110.14159848961611077No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT15780.13869796189585523No Hit
GTGCAGCATGGGATGACAGCCTGTATGGTGTGGTCTTCGGCGGGGGGACC15710.13808269844004345No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAAGACCACACCATACAGGC15460.13588532895500138No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT15320.13465480204337782No Hit
CTCTTGTTCTGGGGGCACCTCCAACATCGGAAGTAATACTGTAAACTGGT15200.13360006469055763No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15190.13351216991115594No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA15090.13263322211713913No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14910.13105111608790884No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG14420.1267442718972264No Hit
GTTTATTACTGTGCGAGACGACATAGTGGGAACTTCTACGGCTGGTTCGA14130.12419532329457761No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13980.12287690160355236No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13710.12050374255970693No Hit
CCTCATGTATAATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATTCT13620.11971268954509177No Hit
TAATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA13400.11777900439825476No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT13300.11690005660423793No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCCGCC13180.11584531925141774No Hit
GCATGGGATGACAGCCTGTATGGTGTGGTCTTCGGCGGGGGGACCAAGCT13130.11540584535440933No Hit
ACTGTAAACTGGTACCAGCAACTCCCGGGAACGGCCCCCAAACTCCTCAT13060.11479058189859755No Hit
CCTGTATGGTGTGGTCTTCGGCGGGGGGACCAAGCTGACCGTCCTAGGTC12870.11312058108996557No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTG12730.11189005417834201No Hit
GAAGTAATACTGTAAACTGGTACCAGCAACTCCCGGGAACGGCCCCCAAA12680.1114505802813336No Hit
TTCCAGGCCACTGTCACGACTCCCGGGTAGAAGTCACTTATGAGACACAC12530.11013215859030838No Hit
TTATTATTATACATGAGGAGTTTGGGGGCCGTTCCCGGGAGTTGCTGGTA12520.11004426381090669No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12200.10723163087005284No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12080.10617689351723265No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11980.10529794572321582No Hit
CTCCTCATGTATAATAATAATCTGCGGCCCTCAGGGGTCCCTGACCGATT11730.10310057623817376No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11570.10169425976774683No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11520.10125478587073843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCA400.00621860454.0009579
GTATCAA15600.053.5818251
GGTATCA5550.053.232271
ATCAACG15100.052.9583663
TCAACGC15300.052.2638054
TATCAAC16200.049.809332
CAACGCA16800.047.5973935
AACGCAG17300.045.781166
CGCAGAG18600.042.58148
GTGCTAG4900.041.176181
TTGAGGG8250.037.527947
GAATACG4450.037.214149
GTACATG28250.036.730611
ACATGGG28050.036.213863
TAGCGCG800.002106915536.000649
GGCAGCG4600.036.0006377
TATTGAG16700.035.803965
TACATGG28850.035.4609382
ATTGAGG8550.035.369056
GCAGAGT22650.035.2853939
Produced by FastQC (version 0.11.5)