FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828864
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCGCGGCGGTCAAAGAACTCAGTCTCAGGGAGA35000.42226468998532934No Hit
GTGTATTACTGTGCGATCGGGATTGTGATTTTTGGAGTGGATGGTGGGGC26250.31669851748899697No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17740.2140278742954212No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17650.2129420508068875No Hit
GTATTACTGTCACCAGTATGGTAGCTCCCCTCAGACGTTCGGCCAAGGGA17230.20787487452706357No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15560.18772681646204928No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14550.17554146397961548No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13590.1639593467685893No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12550.15141205312331094No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12030.14513840630067176No Hit
GTATGGTAGCTCCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCA11960.1442938769207011No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11670.14079511234653694No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11390.13741699482665432No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11100.13391823025249017No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT11000.13271175970967491No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.1313846421125782No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10710.12921299513551077No Hit
GTATTACTGTGCGATCGGGATTGTGATTTTTGGAGTGGATGGTGGGGCCG10110.1219741718786194No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9160.11051270172187475No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9050.10918558412477801No Hit
ATACACAGCCGTGTCCGCGGCGGTCAAAGAACTCAGTCTCAGGGAGAACT9010.10870299590765191No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCT8950.10797911358196277No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCAAAGAACTCAGTCTCAGGGA8840.10665199598486604No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8690.1048422901706432No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8430.1017054667593236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)