FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828864
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCGCGGCGGTCAAAGAACTCAGTCTCAGGGAGA34640.4179213960311945No Hit
GTGTATTACTGTGCGATCGGGATTGTGATTTTTGGAGTGGATGGTGGGGC26640.32140375260597637No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19590.23634757933750292No Hit
GTATTACTGTCACCAGTATGGTAGCTCCCCTCAGACGTTCGGCCAAGGGA17170.2071509922013744No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17080.20606516871284072No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15320.1848312871592927No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14450.17433499343680023No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12870.15527275886031966No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12220.1474307003320207No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12120.14622422978920546No Hit
GTATGGTAGCTCCCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCA11860.1430874063778859No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11530.13910605358659564No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11260.13584858312099451No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10990.13259111265539342No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10280.12402517180140529No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT10150.12245676009574549No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10000.12064705428152267No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9630.11618311327310632No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9620.11606246621882481No Hit
GTATTACTGTGCGATCGGGATTGTGATTTTTGGAGTGGATGGTGGGGCCG9550.11521793683885413No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9180.1107539958304378No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCT8710.10508358427920625No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8650.10435970195351711No Hit
ATACACAGCCGTGTCCGCGGCGGTCAAAGAACTCAGTCTCAGGGAGAACT8430.1017054667593236No Hit
CAGTAATACACAGCCGTGTCCGCGGCGGTCAAAGAACTCAGTCTCAGGGA8380.101102231487916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)