FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972843
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35660.36655452113033654No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29150.29963724876470305No Hit
GTTATGAAGAGTACTACTACAACGGTTTGGACGTCTGGGGCCCAGGGACC25830.26551046777332005No Hit
CTTCATAACCATTCAAAATTCGGTCTCGACTCTCTCTCACACAGTAATAA23880.24546612351633307No Hit
GTAATAAACGGCCGTGTCTTCGGCTCTCAGGCTGCTCATTTCCAGAATCA20660.21236725761505199No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT20340.20907792932672592No Hit
GTTCTATGTGGACTCTGTGAAGGGGCGATTTACCATCTCCAGAGACAACT19870.20424672840324698No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19280.19818202937164578No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18730.19252849637608535No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG17340.178240476623669No Hit
ACTTATTACTGTCAACATTATAATAATTATGCGATCAGCTTCGGCCAAGG17060.17536231437138367No Hit
GGTATCAGCAGCGACCAGGGAGAGCTCCTAACCTCCTGATCTATGATGCC16820.17289531815513912No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15790.1623077927270896No Hit
GTTTATTACTGTGTGAGAGAGAGTCGAGACCGAATTTTGAATGGTTATGA15740.1617938351820386No Hit
GTGTGAGAGAGAGTCGAGACCGAATTTTGAATGGTTATGAAGAGTACTAC15730.16169104367302845No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15600.16035475405589597No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15180.156037510677468No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14950.15367330597023363No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14920.15336493144320307No Hit
GTACTACTACAACGGTTTGGACGTCTGGGGCCCAGGGACCAACGTCACCG14850.15264539088013174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14850.15264539088013174No Hit
GTCTGCATATGTGGGAGAGAGAGTCACCATCACTTGCCGGGCAAGCCAGG14550.14956164560982604No Hit
GTCTCCATCCTCCCTGTCTGCATATGTGGGAGAGAGAGTCACCATCACTT14090.14483323619535732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.14431927865030636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13940.14329136356020447No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13820.14205786545208218No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13700.14082436734395992No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13640.14020761828989878No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13320.1369182900015727No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12620.1297228843708594No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12600.12951730135283906No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12500.12848938626273715No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12310.12653634759154356No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12050.12386376835727862No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12050.12386376835727862No Hit
CTCTCACACAGTAATAAACGGCCGTGTCTTCGGCTCTCAGGCTGCTCATT12030.12365818533925824No Hit
GTACATGGGAGGTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTT11870.1220135211950952No Hit
GTAATAAGTTGCAAAGTCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA11830.12160235515905445No Hit
CTCCTGTGAAGCCTCTGGATTCTCTTTCAGTTACTATTGGATGAGTTGGG11830.12160235515905445No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11810.12139677214103406No Hit
ACATAAAGCAGGATGGAGGTGAGCAGTTCTATGTGGACTCTGTGAAGGGG11710.12036885705093217No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11410.11728511178062646No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11340.11656557121755515No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11050.11358461745625964No Hit
GTCCAGGGAAGGGACTGGAGTGGGTGGCCAACATAAAGCAGGATGGAGGT11040.11348182594724945No Hit
ACCAGGGAGAGCTCCTAACCTCCTGATCTATGATGCCTCCACTTTGCAAA10900.1120427448211068No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10770.11070645520397435No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10720.11019249765892337No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10680.10978133162288263No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG10600.1089589995508011No Hit
CTGTGAAGGGGCGATTTACCATCTCCAGAGACAACTCCAAGAAGTCACTG10470.10762270993366864No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10450.10741712691564825No Hit
CTCCAGAGACAACTCCAAGAAGTCACTGATTCTGGAAATGAGCAGCCTGA10300.10587525428049542No Hit
TAGTAGTACTCTTCATAACCATTCAAAATTCGGTCTCGACTCTCTCTCAC10290.10577246277148522No Hit
GATCTATGATGCCTCCACTTTGCAAAGTGGAGTCCCATCAAGGTTCAGCG10280.10566967126247505No Hit
CCCTGAGACTCTCCTGTGAAGCCTCTGGATTCTCTTTCAGTTACTATTGG10270.10556687975346483No Hit
CTATTGGATGAGTTGGGTCCGCCAGGGTCCAGGGAAGGGACTGGAGTGGG10210.10495013069940372No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10190.10474454768138333No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10090.10371663259128143No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10000.10279150901018971No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9970.10248313448315916No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT9880.10155801090206745No Hit
GGACTGGAGTGGGTGGCCAACATAAAGCAGGATGGAGGTGAGCAGTTCTA9860.10135242788404707No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9760.10032451279394516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21050.063.3819241
ATCAACG20600.063.3041763
CAACGCA21000.062.095195
TATCAAC21250.062.0459182
TCAACGC21300.061.2237634
AACGCAG22600.057.6427236
CGCAGAG25850.050.6748
ACGCAGA26750.048.7000247
GCAGAGT29350.044.3858769
TATATCG657.567973E-444.3348585
ACCGTCG657.6044805E-444.291568
GTACATG25050.041.4572561
TACATGG25200.040.5983922
GGTACGA750.001529673938.4255183
GAATACG3700.036.959519
TAATTGC2151.2732926E-1136.82386
GTATAGG1051.9120658E-434.342071
GTGCTAG4250.033.9380461
TATCGCT850.002841635833.8700147
ATACCGT850.002841635833.8700146
Produced by FastQC (version 0.11.5)