FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972843
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32680.3359226514453No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24520.2520447800929852No Hit
GTTATGAAGAGTACTACTACAACGGTTTGGACGTCTGGGGCCCAGGGACC22810.23446743205224277No Hit
CTTCATAACCATTCAAAATTCGGTCTCGACTCTCTCTCACACAGTAATAA22090.22706644340350907No Hit
GTAATAAACGGCCGTGTCTTCGGCTCTCAGGCTGCTCATTTCCAGAATCA20100.20661093311048132No Hit
GTTCTATGTGGACTCTGTGAAGGGGCGATTTACCATCTCCAGAGACAACT18950.19478990957430953No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18860.19386478599321783No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT17720.1821465539660562No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17020.17495114833534292No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG15920.16364408234422204No Hit
GTTTATTACTGTGTGAGAGAGAGTCGAGACCGAATTTTGAATGGTTATGA15720.16158825216401823No Hit
ACTTATTACTGTCAACATTATAATAATTATGCGATCAGCTTCGGCCAAGG15660.16097150310995711No Hit
GGTATCAGCAGCGACCAGGGAGAGCTCCTAACCTCCTGATCTATGATGCC15160.15583192765944762No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14710.15120630975398908No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14240.14637510883051016No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14050.14442207015931657No Hit
GTGTGAGAGAGAGTCGAGACCGAATTTTGAATGGTTATGAAGAGTACTAC13690.1407215758349497No Hit
GTCTGCATATGTGGGAGAGAGAGTCACCATCACTTGCCGGGCAAGCCAGG13630.1401048267808886No Hit
GTACTACTACAACGGTTTGGACGTCTGGGGCCCAGGGACCAACGTCACCG13610.13989924376286822No Hit
GTCTCCATCCTCCCTGTCTGCATATGTGGGAGAGAGAGTCACCATCACTT13370.13743224754662364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12950.1331150041681957No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12930.1329094211501753No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12760.1311619654970021No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12760.1311619654970021No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12170.1250972664654009No Hit
GTAATAAGTTGCAAAGTCCTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA12100.12437772590232957No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12020.12355539383024805No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11920.12252747874014615No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11790.12119118912301367No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11780.1210883976140035No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11490.118107443852708No Hit
CTCTCACACAGTAATAAACGGCCGTGTCTTCGGCTCTCAGGCTGCTCATT11490.118107443852708No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11340.11656557121755515No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11280.115948822163494No Hit
GTACATGGGAGGTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTT11270.11584603065448382No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11010.11317345142021888No Hit
GTCCAGGGAAGGGACTGGAGTGGGTGGCCAACATAAAGCAGGATGGAGGT10950.11255670236615775No Hit
CTCCTGTGAAGCCTCTGGATTCTCTTTCAGTTACTATTGGATGAGTTGGG10940.11245391085714755No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10780.11080924671298452No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10650.10947295709585204No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10390.10680037786158714No Hit
CTCCAGAGACAACTCCAAGAAGTCACTGATTCTGGAAATGAGCAGCCTGA10280.10566967126247505No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10250.10536129673544446No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10230.10515571371742408No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10160.10443617315435275No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT10160.10443617315435275No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9970.10248313448315916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13800.055.2264144
ATCAACG13950.055.139473
GTATCAA14700.054.3550871
TATCAAC14650.053.979692
CAACGCA14100.053.5497325
AACGCAG15050.050.185026
GAATACG3400.048.659799
GTGCTAG3600.047.9891781
CGCAGAG17950.042.4778678
GTACATG27650.040.6129041
TACATGG27600.040.113172
ACGCAGA19600.038.9019247
GCAGAGT20700.036.8346759
TACACGG4100.033.334286
GTACACG4050.031.9598435
ACATGGG34050.031.8795973
AGGGGTG6800.031.7248445
GCCTAAC1153.2973458E-431.297291
CTAGAGA5800.029.7511184
TGCTAGA5850.029.4938012
Produced by FastQC (version 0.11.5)