FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784644
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTATAGTATTAGCAACAAGTACTCCTGGACCCCTGCCCGGTTCTCAG24080.3068907682974699No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA22350.2848425528010155No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT21340.2719704732337213No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG20030.25527500369594364No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC19310.2460988677667834No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17210.21933513797339937No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA16920.21563919433526543No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15860.20212988310622398No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15090.1923165155153165No Hit
CTATAGATAAGTGCCCTGGGTGCTTGCCCAGGTTTCTGCTGGAACCAGTT14860.18938524987127922No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14380.1832678259185057No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCCGACAGGGTCAGGGCAG14270.1818659162626618No Hit
TCGTAGTAGAGCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACC14090.17957188228037171No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13530.1724348876688027No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13300.16950362202476538No Hit
CTTATCTATAGTATTAGCAACAAGTACTCCTGGACCCCTGCCCGGTTCTC13190.16810171236892144No Hit
TCCTCAGGCTGCACACCCGACAGGGTCAGGGCAGCTTTGCCCCCAAGGAG12440.15854323744271287No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12360.1575236667839173No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA12100.15421006214283167No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11910.15178858182819216No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11690.1489847625165043No Hit
GACTATTACTGCCTGCTCTACTACGATGGTGCTCAGCCTTGGGTGTTCGG11610.14796519185770873No Hit
GTCCAGGAGTACTTGTTGCTAATACTATAGATAAGTGCCCTGGGTGCTTG11510.14669072853421425No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG11270.1436320165578275No Hit
GACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGGCTGAGCA11230.1431222312284297No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11190.14261244589903194No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11180.14248499956668248No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11160.14223010690198357No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11090.14133798257553745No Hit
ACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGGCTGAGCAC10790.13751459260505403No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10700.136367575613909No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10530.13420098796396837No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10440.13305397097282334No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10430.1329265246404739No Hit
TAGTAGAGCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCCGA10350.13190695398167832No Hit
CTACTACGATGGTGCTCAGCCTTGGGTGTTCGGCGGAGGGACCAAGTTGA10210.13012270532878606No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC10200.1299952589964366No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10160.1294854736670388No Hit
GTATTAGCAACAAGTACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTC10100.12872079567294212No Hit
ATAGATAAGTGCCCTGGGTGCTTGCCCAGGTTTCTGCTGGAACCAGTTTG10090.1285933493405927No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9730.12400528137601256No Hit
GCCTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCA9730.12400528137601256No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9670.12324060338191588No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9430.12018189140552914No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9350.11916232074673355No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9080.11572126977329845No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTGCTCTACTACGATGGTGCTC8810.11228021879986338No Hit
GTACATGGGGGCGCCAGGGGTCCCTTCCAATATCAGCACCATGGCCTGGA8790.11202532613516449No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8550.10896661415877773No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC8480.10807448983233162No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8360.10654513384413823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.10641768751178879No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTGTCGGGTG8290.1056530095176921No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8280.10552556318534265No Hit
ATCCAAACTGGTTCCAGCAGAAACCTGGGCAAGCACCCAGGGCACTTATC8130.10361386820010093No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8100.10323152920305259No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG7880.10042770989136475No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)