FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784644
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTATAGTATTAGCAACAAGTACTCCTGGACCCCTGCCCGGTTCTCAG21500.27400961455131245No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA19300.24597142143443396No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT18170.23156998587894637No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG18080.23042296888780134No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC17390.22162917195568946No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15910.2027671147679712No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA15200.19371842517116042No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA13590.17319956566289935No Hit
CTATAGATAAGTGCCCTGGGTGCTTGCCCAGGTTTCTGCTGGAACCAGTT12930.1647881077278358No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12800.16313130540729298No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12360.1575236667839173No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCCGACAGGGTCAGGGCAG12220.15573941813102501No Hit
CTTATCTATAGTATTAGCAACAAGTACTCCTGGACCCCTGCCCGGTTCTC12050.15357283048108442No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11530.14694562119891313No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11460.146053496872467No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10960.13968118025499462No Hit
TCGTAGTAGAGCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACC10920.13917139492559683No Hit
GTCCAGGAGTACTTGTTGCTAATACTATAGATAAGTGCCCTGGGTGCTTG10920.13917139492559683No Hit
TCCTCAGGCTGCACACCCGACAGGGTCAGGGCAGCTTTGCCCCCAAGGAG10520.13407354163161891No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10080.12846590300824323No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9770.12451506670541036No Hit
GACTATTACTGCCTGCTCTACTACGATGGTGCTCAGCCTTGGGTGTTCGG9750.12426017404071146No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9630.12273081805251808No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9260.11801530375558852No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG9220.11750551842619072No Hit
GACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGGCTGAGCA9190.11712317942914238No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9080.11572126977329845No Hit
ACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAGGCTGAGCAC9060.11546637710859955No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA9010.11482914544685233No Hit
GTATTAGCAACAAGTACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTC8900.11342723579100841No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8850.11279000412926117No Hit
GCCTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCA8800.11215277246751393No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8500.10832938249703052No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8430.10743725817058437No Hit
ATAGATAAGTGCCCTGGGTGCTTGCCCAGGTTTCTGCTGGAACCAGTTTG8430.10743725817058437No Hit
TAGTAGAGCAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACCCGA8420.10730981183823493No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8200.10450599252654706No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8200.10450599252654706No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8180.10425109986184818No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8150.10386876086479982No Hit
GCCTGAGGACGAGGCTGACTATTACTGCCTGCTCTACTACGATGGTGCTC8110.10335897553540203No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8070.10284919020600425No Hit
CTACTACGATGGTGCTCAGCCTTGGGTGTTCGGCGGAGGGACCAAGTTGA7940.10119238788546144No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC7900.10068260255606365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG10450.048.2185863
TCAACGC10900.047.548714
GAATACG2600.044.2975169
CAACGCA11950.042.7684175
TATCAAC12000.041.390492
GTATCAA12600.040.6061021
AACGCAG13000.040.4214826
GTACATG20200.039.241431
GGTATCA5900.037.862841
TACATGG20200.037.417152
CGCAGAG14250.035.8654448
ACATGGG21000.034.9633983
TCTACGA1051.9328765E-434.277842
GTGCTAG3800.034.134411
TAGACGG5100.032.463137
CATGGGG13550.032.4058384
TCTACAC10400.031.8388393
AGACGGC5400.030.6596228
GCAGAGT16750.030.0826449
CTAGAGA4100.029.8468024
Produced by FastQC (version 0.11.5)