FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672962
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATATACTGATGGAAACACCTTCTTGCATTGGTTTCAGCAGAGGCCGG23900.3551463529887275No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21830.32438681530309293No Hit
ATATAGGGCCGTGTCGTCGGCGCTCAGGCTGCTCATGTGCAAGTAGAGCG19110.28396848559056825No Hit
CCCTTGGACAGTCGGCCTCCATCTCCTGTTGGTCTAGTCAAAGCCTCGTA18590.2762414519690562No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16630.2471164790879723No Hit
GCCCTATATTTCTGTGCAAAAGACCCCAACTGGGACGACGGCCAGTGGGG14970.22244941021929915No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14970.22244941021929915No Hit
GCTACACACTGGCCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAA12730.1891637269266318No Hit
GAAATATAGGGCCGTGTCGTCGGCGCTCAGGCTGCTCATGTGCAAGTAGA12620.18752916212208118No Hit
GTGTGTAGCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACCC12510.18589459731753055No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12390.18411143571256625No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG10950.16271349645299438No Hit
GTATGCTGGTTACAGCGTAGGTGGTGGTGCCCTGGCTGGGCTCCTGCTGG10810.16063314124720268No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10690.15884997964223835No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT10440.155135059631896No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10370.15409488202900015No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10290.15290610762569062No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT10210.15171733322238107No Hit
GTCACATCCTGGCTGGAATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9480.14086976679218144No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT9430.14012678279011298No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9380.1393837987880445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.13730344358225277No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9170.13626326597935692No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCATCCGCCT9160.13611466917894324No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8870.13180536196694614No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8850.13150816836611873No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8850.13150816836611873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8680.12898202275908593No Hit
GCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGG8580.12749605475494902No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8560.12719886115412163No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGCCTTTA8380.12452411874667516No Hit
TTATTATTGCATGCAAGCTACACACTGGCCGGGGACGTTCGGCCAAGGGA8110.12051200513550542No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT7870.11694568192557678No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7610.11308216511482076No Hit
CTTATACCATGATGTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTTC7470.11100180990902904No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC7290.10832706750158255No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCACTTATACCATGATGTGGG7250.10773268029992777No Hit
TCTCTGGAGACGGTGAAGCGGCCCTTCACGGAGTTTGCGTAGGAGGCGTC7250.10773268029992777No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA7040.10461214749124023No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7000.10401776028958545No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT6980.10372056668875805No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6920.10282898588627591No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGTACTGAGTTCACACTGAA6870.10208600188420744No Hit
CTATATTTCTGTGCAAAAGACCCCAACTGGGACGACGGCCAGTGGGGCCA6800.10104582428131159No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6790.10089722748089788No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC6780.10074863068048419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4750.077.4118351
GTATCAA14700.065.232661
ATCAACG15750.058.991253
TCAACGC15800.058.8045654
CAACGCA16400.056.648975
TATCAAC16600.056.40452
AACGCAG16750.055.8626
ACGCAGA19850.047.1344577
CGCAGAG20150.046.4327058
GCAGAGT22850.041.2610939
GTACATG20000.039.654591
TACATGG20250.038.057232
GTGCTAG4300.036.887991
ACATGGG21100.036.1827773
ACCGACC3250.035.4317259
GCACCGA3100.034.8245287
GACTGCG1051.9346016E-434.2717557
GTGTAGA3700.031.1780571
CCTATAC1402.8716475E-530.8675163
TGCACCG3500.030.8468726
Produced by FastQC (version 0.11.5)