FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672962
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21000.31205328086875633No Hit
CGTATATACTGATGGAAACACCTTCTTGCATTGGTTTCAGCAGAGGCCGG19540.2903581480083571No Hit
ATATAGGGCCGTGTCGTCGGCGCTCAGGCTGCTCATGTGCAAGTAGAGCG16180.24042962306935609No Hit
CCCTTGGACAGTCGGCCTCCATCTCCTGTTGGTCTAGTCAAAGCCTCGTA15860.2356745254561179No Hit
GCCCTATATTTCTGTGCAAAAGACCCCAACTGGGACGACGGCCAGTGGGG15040.22348958782219502No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14970.22244941021929915No Hit
GTGTGTAGCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACCC13090.19451321174152478No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12470.18530021011587577No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12390.18411143571256625No Hit
GCTACACACTGGCCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAA11610.17252088528029813No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11280.16761719086664625No Hit
GTCATGGAACCATGGGGAGACCTTCACCTGCACTGCTGCCCACCCCGAGT11010.16360507725547654No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG10460.1554322532327234No Hit
GAAATATAGGGCCGTGTCGTCGGCGCTCAGGCTGCTCATGTGCAAGTAGA10350.15379768842817276No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10000.1485968004136935No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9950.14785381641162504No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9680.1438417028004553No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT9110.13537168517687478No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT9100.13522308837646108No Hit
GCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGG9060.1346287011748063No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8790.13061658756363656No Hit
TCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8700.12927921635991335No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8500.1263072803516395No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCGCCTTTA8390.12467271554708884No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8180.1215521827384013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8080.12006621473426435No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7660.11382514911688921No Hit
CTTATACCATGATGTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTTC7540.11204198751192489No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7400.10996163230613319No Hit
TTATTATTGCATGCAAGCTACACACTGGCCGGGGACGTTCGGCCAAGGGA7270.10802987390075516No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7260.10788127710034147No Hit
TCTCTGGAGACGGTGAAGCGGCCCTTCACGGAGTTTGCGTAGGAGGCGTC7210.107138293098273No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCACTTATACCATGATGTGGG7040.10461214749124023No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7010.10416635708999913No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA6940.10312617948710329No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC6930.10297758268668958No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6860.10193740508379373No Hit
CTCCAGAGACGACTCGAAGAATACGCTCTACTTGCACATGAGCAGCCTGA6810.10119442108172526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3250.066.439171
CAACGCA9750.064.9144065
ATCAACG9700.064.493153
TCAACGC9700.063.7565964
AACGCAG10800.058.603286
TATCAAC11150.056.106152
GTATCAA11850.054.665141
ACGCAGA12600.050.80227
CGCAGAG12800.048.8882688
GCAGAGT14450.042.8080989
GTGCTAG4050.039.0979461
GGGCCGT10850.035.1325076
ATAGGGC11750.034.8821373
CTAGAGA4950.034.8661844
TAGGGCC11750.034.2727174
GTCTTAG1501.199287E-633.5886881
GGCCGTG11450.033.29157
TATAGGG12300.032.7377622
CCGTGTC11450.032.665799
GTACATG17500.032.4919171
Produced by FastQC (version 0.11.5)