FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032366
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16750.16224865987450188No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16010.15508065937855373No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15720.1522715781031146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.13987287454255565No Hit
GCTATGCAGTGGGTGCGACAGACTCGTGGACAACGCCTTGAGTGGATAGG14190.137451252753384No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12250.11865946766941181No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG11920.11546292690770522No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11360.11003849409996068No Hit
CTACTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCG11170.10819806154019021No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10700.10364541257654747No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10600.1026767638608788No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT10430.10103006104424206No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10360.10035200694327398No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)