FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032366
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19050.18452758033488123No Hit
GTAATATGCGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTAGG18980.18384952623391315No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16400.15885838936966154No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16120.15614617296578925No Hit
CCGTAGTAGTAGTAGTAGCAGTTGCCACCACTACAATGACTGCCCCTCGA12130.1174970892106094No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11280.10926357512742574No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11150.10800433179705647No Hit
CTACTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCG11070.10722941282452153No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10910.10567957487945168No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10720.10383914231968121No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10620.10287049360401253No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10470.10141752053050954No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG259.6467446E-486.403877
TACGCGA350.003666419361.7110638
CTAGCGC502.0752812E-457.627723
CTAGCGT450.00988755948.0230984
TAGCGCT850.002829963333.898664
GTACATG35750.030.634751
TAGCGTG950.004878612730.3303815
TACCGCA1804.958396E-628.0012537
GTATCAA22500.027.5399231
GTACACG8650.027.481435
TCAACGC21550.027.075673
TACACGG8850.026.848666
ACATGGG40550.025.580743
ATCAACG22600.025.5014652
TACACCG1701.0774498E-425.4239965
CAACGCA23100.025.2589044
TATCAAC23400.024.941121
TTTCGCG2051.3624589E-524.5828919
ACACGGG10000.024.4810967
ACGGGGT9850.024.11949
Produced by FastQC (version 0.11.5)