FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712079
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCATCTCTCAGGCTGTTCACTTGCAGATAGA29120.4089433897081644No Hit
GTTTATTACTGTGCGAAAAGGGGTGGTGACGACTATAGAAGCTCGTCCTC20630.2897150456620684No Hit
GTATTTACTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT17800.24997226431336972No Hit
AGTAAATACTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA17510.24589968247905078No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG15220.21374032937356668No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14820.20812297511933367No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13740.1929561186329045No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11970.16809932605792335No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11940.16767802448885588No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11120.15616244826767817No Hit
ATACTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT10590.14871945388081942No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10490.14731511531726116No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10310.14478730590285627No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9650.13551867138337179No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9320.13088435412362953No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9030.1268117722893106No Hit
GCATAGTATTTACTACTTCCATCATATGATATAACTGCCACCCACTCCAG8700.12217745502956835No Hit
CTATAGAAGCTCGTCCTCGTACTTCCAACACTGGGGCCAGGGCACCCTGG8570.12035181489694262No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8390.11782400548253774No Hit
GTCTATCTGCAAGTGAACAGCCTGAGAGATGAGGACACGGCTGTTTATTA8360.11740270391347027No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGA8300.11656010077533531No Hit
GATGTACACTGGTACCAGCAGGTTCCAGGAACATCCCCCAAACTCCTCAT8090.11361098979186299No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8000.11234708508466056No Hit
CTTCTATAGTCGTCACCACCCCTTTTCGCACAGTAATAAACAGCCGTGTC7750.10883623867576492No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7520.10560625997958091No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAG7390.10378061984695518No Hit
GTGTTGGAAGTACGAGGACGAGCTTCTATAGTCGTCACCACCCCTTTTCG7340.10307845056517606No Hit
GCCTGAGAGATGAGGACACGGCTGTTTATTACTGTGCGAAAAGGGGTGGT7320.1027975828524644No Hit
CTCTCAGGCTGTTCACTTGCAGATAGACCGTGTTCTTGGAATTGTCTCTG7150.10041020729441537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCGT14350.034.116979
CCGTAAT900.003745634632.012081
TAAACAG16600.030.3601115
GGTTTAT5150.029.370311
TATTGAG6950.029.0059225
TTATTGA7750.027.8697434
GTATGTA8350.027.6032331
CATGGGG9950.027.496284
GTACACG6200.026.7085065
AAACAGC18650.026.2508566
CAGCGCT2757.2777766E-926.180679
ACAGCCG24250.025.53298
TACACGG6550.025.2813326
ATTGAGT5750.025.042386
CCTAGTA1450.001276891124.8264962
ACACGGG7000.024.6846337
GTACTGT3052.1884262E-823.6055226
ATAAACA20200.023.523724
CTGTGCG16900.023.4309239
AATAAAC19900.023.1547623
Produced by FastQC (version 0.11.5)