FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712079
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCATCTCTCAGGCTGTTCACTTGCAGATAGA30050.4220037383492562No Hit
GTTTATTACTGTGCGAAAAGGGGTGGTGACGACTATAGAAGCTCGTCCTC20920.29378762749638737No Hit
GTATTTACTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT17180.24126536521930855No Hit
AGTAAATACTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGA16820.23620974639049883No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15800.2218854930422046No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13970.19618609732908848No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG13810.19393915562739528No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12910.18130010855537096No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11300.15869025768208303No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10350.14534904132827958No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10320.1449277397592121No Hit
ATACTATGCAGACTCCGTGGAGGGCCGATTCACCATCTCCAGAGACAATT10220.14352340119565385No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10040.14099559178124899No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9670.13579953909608344No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9420.1322886926871878No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8780.12330092588041495No Hit
GCATAGTATTTACTACTTCCATCATATGATATAACTGCCACCCACTCCAG8170.11473446064270959No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8110.11389185750457463No Hit
CTTCTATAGTCGTCACCACCCCTTTTCGCACAGTAATAAACAGCCGTGTC7980.1120662173719489No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7850.11024057723932318No Hit
CTATAGAAGCTCGTCCTCGTACTTCCAACACTGGGGCCAGGGCACCCTGG7820.10981927567025569No Hit
GTCTATCTGCAAGTGAACAGCCTGAGAGATGAGGACACGGCTGTTTATTA7660.10757233396856247No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7600.10672973083042751No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7380.10364018599059936No Hit
GATGTACACTGGTACCAGCAGGTTCCAGGAACATCCCCCAAACTCCTCAT7140.10026977343805955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG1557.894414E-1041.8183671
TACGAGT1752.59206E-937.0365224
TACACCG800.002105804636.0026665
ATACGAG1905.7880243E-934.1125873
ACGAGTG1957.4687705E-933.2332345
GTATTAG2900.032.2850571
CAGCCGT13800.031.302279
TCTAAGC2901.2732926E-1129.79953
TAAACAG17150.028.550225
TATACGA2303.7192876E-828.1799622
GTCTAGT1804.9463106E-628.007981
GTTAGAC1050.00796332727.434463
GTACATG36050.026.3708951
CATGGGG10400.026.313344
TACATGG36150.026.2961032
CTACTAG1100.0099875726.189281
TAGGAGT2207.9317215E-726.187444
GTGCGTG1400.00104074825.7125829
ACATGGG36950.025.5318663
CTGTGCG14700.024.488179
Produced by FastQC (version 0.11.5)