FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399077
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC28700.20513524273503173No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23230.16603803793501No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT18800.1343743053455957No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG14640.10464041650316601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21850.070.5116351
TCAACGC24650.061.254894
CAACGCA25050.060.000475
ATCAACG25200.059.915833
AACGCAG27050.056.1019486
CGCAGAG29950.050.6678928
GGTATCA10250.048.4644931
TATCAAC34450.044.4542432
GCAGCGC11850.044.3048938
GCAGAGT37600.040.7416849
GGCAGCG12900.040.7001387
CAGCGCT13500.039.955339
ACGCAGA39100.038.8122147
GTACACG18300.031.4380075
TACACGG18550.030.6299256
GTACATG47200.030.048491
TACATGG46000.029.8537122
ACATGGG46400.029.2864443
CATGGGT15500.027.3689824
CAGGGCA23300.027.1559584
Produced by FastQC (version 0.11.5)