FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463952
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCATGTAGG18560.40004138359140595No Hit
ATATATTACTGTGCGGAGGGCTATCACGATATTCAGACTGGTTATTACCA15620.336672759250957No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA9570.20627133841431872No Hit
GTATTACTGTCAGCAGTATGGTAGCTCATTTTGGACGTTCGGCCAAGGGA9270.19980515225713003No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9190.19808083594854642No Hit
ATATATGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCATGTAGGCTG9170.1976497568714005No Hit
GGTTATTACCATGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC9040.19484774286995207No Hit
ATATTACTGTGCGGAGGGCTATCACGATATTCAGACTGGTTATTACCATG8110.17480256578266717No Hit
GCCATATATTACTGTGCGGAGGGCTATCACGATATTCAGACTGGTTATTA8100.1745870262440942No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8070.17394040762837537No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC7870.16962961685691622No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7710.16618098423974895No Hit
GTAATAACCAGTCTGAATATCGTGATAGCCCTCCGCACAGTAATATATGG7630.1644566679311653No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7560.1629478911611546No Hit
GCCTACATGGAGTTGAGCAGCCTGAGATCTGAGGACACGGCCATATATTA7110.1532486119253716No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6910.14893782115391246No Hit
CAGTAATATATGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCATGTA6830.14721350484532883No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT6440.13880746284098355No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6240.13449667206952443No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6220.13406559299237852No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6030.12997034175949237No Hit
GTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTGGAGTGGATGGGA5990.12910818360520054No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5920.12759940683518986No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTTATCGTGACTCTGCCC5690.12264199744801187No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5600.12070214160085527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.1150981135979584No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG5240.11294271821222886No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5100.10992516467220746No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT4870.10496775528502948No Hit
CATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTTATCGTGACTCTGCCCT4750.102381280822154No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4700.10130358312928923No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT4700.10130358312928923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC10000.058.3232274
ATCAACG10100.057.7457733
CAACGCA10250.056.894585
GTATCAA10800.056.6942521
AACGCAG10450.055.8056876
TATCAAC10800.054.6696972
CGCAGAG12300.047.4121488
ACGCAGA14800.039.4033437
ATAGGAC800.002105243736.0019953
ATGGCCG9450.034.283928
TGGCCGT9650.033.573379
GTAATAT10100.033.521261
TATGGCC9750.033.229037
ATATGGC9800.033.059496
AGCGCTG2950.031.727155
TAATATA10600.031.2470112
TGACCTA1652.5159316E-630.5438565
CAGCGCT2901.2732926E-1129.7947524
GCAGAGT20600.029.707189
GTACATG12300.029.2825371
Produced by FastQC (version 0.11.5)