FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636821
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGAGACCTGAGGACACCGCCTTCTATTATTGTGCAAAAAATGCGAAC24820.3897484536471002No Hit
GTATGTGGTGTCGCCATCCCCTCCAATAAGACAGACCCACTCCAGACCCT20330.3192419847963556No Hit
GTCTCAGACTGTGCATTTGTAGATAGAGGGAGTTTTTGTCGTTGTCTCTG18180.2854805353466673No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17550.27558764550792136No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17450.27401734553351725No Hit
GTCTTATTGGAGGGGATGGCGACACCACATACTATGCAGACTCTGTAAAG15880.24936363593537272No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15590.2448097660096008No Hit
ATAATAGAAGGCGGTGTCCTCAGGTCTCAGACTGTGCATTTGTAGATAGA14240.22361071635514534No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13920.21858575643705214No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13840.21732951645752888No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13400.2104201965701508No Hit
GGTCTCAGACTGTGCATTTGTAGATAGAGGGAGTTTTTGTCGTTGTCTCT13340.20947801658550833No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13120.2060233566418193No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12500.19628749680051383No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12430.19518828681843092No Hit
ACCACATACTATGCAGACTCTGTAAAGGGCAGATTCACCATCTCCAGAGA12170.19110550688498024No Hit
GTATGATAACTGGCCTCCGGACACTTTTGGCCAGGGGACCAAGCTGGAGA12020.18875005692337407No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC11800.18529539697968503No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11730.18419618699760215No Hit
CCACATACTATGCAGACTCTGTAAAGGGCAGATTCACCATCTCCAGAGAC11500.1805844970564727No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCG11120.17461735715373708No Hit
TTTGTAGATAGAGGGAGTTTTTGTCGTTGTCTCTGGAGATGGTGAATCTG10780.1692783372407631No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10650.16723694727403776No Hit
ATACTATGCAGACTCTGTAAAGGGCAGATTCACCATCTCCAGAGACAACG10600.1664517972868357No Hit
ATCTACAAATGCACAGTCTGAGACCTGAGGACACCGCCTTCTATTATTGT10340.16236901735338502No Hit
TTCTATTATTGTGCAAAAAATGCGAACGAAGATATCGAGTCGGGGCCCTT10250.16095574737642132No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10210.16032762738665968No Hit
CTATGCAGACTCTGTAAAGGGCAGATTCACCATCTCCAGAGACAACGACA10010.15718702743785146No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9950.156244847453209No Hit
CAATAATAGAAGGCGGTGTCCTCAGGTCTCAGACTGTGCATTTGTAGATA9810.15404642748904324No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9040.1419551176861316No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8750.13740124776035967No Hit
CTATTATTGTGCAAAAAATGCGAACGAAGATATCGAGTCGGGGCCCTTTG8640.13567391778851512No Hit
GCATAGTATGTGGTGTCGCCATCCCCTCCAATAAGACAGACCCACTCCAG8620.13535985779363433No Hit
GTCTGGGACAGAGTTCACCCTCACCATCGACACCCTGCAGTCTGAAGATG8470.13300440783202816No Hit
GTTATCATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGG8450.1326903478371473No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT8430.13237628784226652No Hit
GTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACC8400.13190519784994528No Hit
CCCTGAGACTCTCCTGCGCAGCCTCTGGATTCGCCTTCCATGATTCTGGC8380.13159113785506446No Hit
GTCTGCATAGTATGTGGTGTCGCCATCCCCTCCAATAAGACAGACCCACT8330.13080598786786238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8320.130648957870422No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8320.130648957870422No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8200.12876459790113703No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCACTGGCCCTCGT8140.12782241791649457No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7820.12279745799840143No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7750.12169824801631857No Hit
CTCCTGCGCAGCCTCTGGATTCGCCTTCCATGATTCTGGCATGCACTGGG7690.12075606803167609No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7680.12059903803423569No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7610.1194998280521528No Hit
TTATTACTGTCAGCAGTATGATAACTGGCCTCCGGACACTTTTGGCCAGG7540.11840061807006992No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7530.11824358807262952No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7530.11824358807262952No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7490.11761546808286788No Hit
CCAATAAGACAGACCCACTCCAGACCCTTTCCTGGAGCTTGACGGACCCA7460.11714437809054665No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7340.11526001812126171No Hit
GTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCGTCGTACAGCCGGGGG7240.11368971814685758No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7180.11274753816221514No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7180.11274753816221514No Hit
GCACTGAACACAGAGAACTCACCATGGAGTTTGGACTGAACTGGGTTTTC7140.11211941817245348No Hit
CTCCCAGACTCCTCATCTATGATACATCCACGAGGGCCAGTGGTATCCCA7090.11133426818525144No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7080.11117723818781101No Hit
GCTCAGCACTGAACACAGAGAACTCACCATGGAGTTTGGACTGAACTGGG7040.11054911819804937No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7020.11023505820316855No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG6980.1096069382134069No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6980.1096069382134069No Hit
GTCTGGGGGAGGCGTCGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGCG6920.10866475822876444No Hit
GAGTTCACCCTCACCATCGACACCCTGCAGTCTGAAGATGTTGCAGTTTA6850.10756554824668156No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC6760.10615227826971786No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTTAGCCTGGTACCAGC6730.10568118827739663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.10552415827995623No Hit
GTCTGTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA6690.10505306828763498No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT6660.10458197829531375No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6620.10395385830555212No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6550.10285464832346923No Hit
CAATAAGACAGACCCACTCCAGACCCTTTCCTGGAGCTTGACGGACCCAG6530.10254058832858842No Hit
GAACTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGC6480.10175543834138635No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC6470.10159840834394596No Hit
GAACACAGAGAACTCACCATGGAGTTTGGACTGAACTGGGTTTTCCTTGT6440.10112731835162471No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG6440.10112731835162471No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA6410.10065622835930348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3900.077.582631
GTATCAA9850.064.361511
TCAACGC11800.052.475614
TATCAAC11850.052.2541922
ATCAACG11900.052.0346343
CAACGCA12050.051.3869065
AACGCAG12800.048.375956
CAGCGCT3450.045.910349
CGCAGAG14900.041.55468
GCAGCGC4000.041.397568
GTCCATA951.0676547E-437.916321
GCAGAGT16950.036.5288249
GTCTAAT800.002100524536.0205081
CCGCAAG800.00210701335.997889
GGCAGCG4450.035.593417
CAGGGCA5350.033.645524
CATTAGC3250.033.2288138
CATGGGA7050.032.681494
ACATGGG14600.032.0554243
GTACATG14850.032.0182271
Produced by FastQC (version 0.11.5)