FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636821
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGAGACCTGAGGACACCGCCTTCTATTATTGTGCAAAAAATGCGAAC24530.3851945837213283No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19920.3128037549012988No Hit
GTATGTGGTGTCGCCATCCCCTCCAATAAGACAGACCCACTCCAGACCCT19880.31217563491153716No Hit
GTCTCAGACTGTGCATTTGTAGATAGAGGGAGTTTTTGTCGTTGTCTCTG18550.29129064525196247No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17510.2749595255181597No Hit
GTCTTATTGGAGGGGATGGCGACACCACATACTATGCAGACTCTGTAAAG16340.2565870158176316No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15590.2448097660096008No Hit
ATAATAGAAGGCGGTGTCCTCAGGTCTCAGACTGTGCATTTGTAGATAGA14630.22973488625532135No Hit
GGTCTCAGACTGTGCATTTGTAGATAGAGGGAGTTTTTGTCGTTGTCTCT13290.20869286659830627No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13200.20727959662134257No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13150.20649444663414052No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12750.2002132467365241No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12430.19518828681843092No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12230.1920476868696227No Hit
GTATGATAACTGGCCTCCGGACACTTTTGGCCAGGGGACCAAGCTGGAGA11770.1848243069873638No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11730.18419618699760215No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11530.18105558704879393No Hit
ACCACATACTATGCAGACTCTGTAAAGGGCAGATTCACCATCTCCAGAGA10770.1691213072433227No Hit
CAATAATAGAAGGCGGTGTCCTCAGGTCTCAGACTGTGCATTTGTAGATA10710.16817912725868023No Hit
CCACATACTATGCAGACTCTGTAAAGGGCAGATTCACCATCTCCAGAGAC10560.16582367729707406No Hit
TTTGTAGATAGAGGGAGTTTTTGTCGTTGTCTCTGGAGATGGTGAATCTG10420.1636252573329083No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10420.1636252573329083No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC10390.16315416734058708No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCG10290.16158386736618296No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10090.15844326741737474No Hit
TTCTATTATTGTGCAAAAAATGCGAACGAAGATATCGAGTCGGGGCCCTT10030.15750108743273228No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9980.1567159374455302No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9970.1565589074480898No Hit
GCATAGTATGTGGTGTCGCCATCCCCTCCAATAAGACAGACCCACTCCAG9580.15043473754791378No Hit
CTATGCAGACTCTGTAAAGGGCAGATTCACCATCTCCAGAGACAACGACA9190.14431056764773775No Hit
ATACTATGCAGACTCTGTAAAGGGCAGATTCACCATCTCCAGAGACAACG9190.14431056764773775No Hit
ATCTACAAATGCACAGTCTGAGACCTGAGGACACCGCCTTCTATTATTGT9100.14289729767077405No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9100.14289729767077405No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCACTGGCCCTCGT9080.14258323767589323No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9080.14258323767589323No Hit
GTCTGCATAGTATGTGGTGTCGCCATCCCCTCCAATAAGACAGACCCACT9030.14179808768869118No Hit
GTCTGGGACAGAGTTCACCCTCACCATCGACACCCTGCAGTCTGAAGATG8730.13708718776547885No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8720.13693015776803844No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8620.13535985779363433No Hit
GTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACC8510.1336325278217898No Hit
CCCTGAGACTCTCCTGCGCAGCCTCTGGATTCGCCTTCCATGATTCTGGC8410.1320622278473857No Hit
GTTATCATACTGCTGACAGTAATAAACTGCAACATCTTCAGACTGCAGGG8370.13143410785762405No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8340.13096301786530282No Hit
CTCCTGCGCAGCCTCTGGATTCGCCTTCCATGATTCTGGCATGCACTGGG8310.13049192787298158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8120.12750835792161377No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT8050.12640914793953087No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7820.12279745799840143No Hit
CTATTATTGTGCAAAAAATGCGAACGAAGATATCGAGTCGGGGCCCTTTG7780.1221693380086398No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7670.12044200803679526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7610.1194998280521528No Hit
CCAATAAGACAGACCCACTCCAGACCCTTTCCTGGAGCTTGACGGACCCA7500.11777249808030829No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7220.11337565815197678No Hit
CTCCCAGACTCCTCATCTATGATACATCCACGAGGGCCAGTGGTATCCCA6990.1097639682108473No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6960.10929287821852607No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC6950.10913584822108567No Hit
TTATTACTGTCAGCAGTATGATAACTGGCCTCCGGACACTTTTGGCCAGG6940.10897881822364526No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6940.10897881822364526No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6920.10866475822876444No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG6860.10772257824412197No Hit
GTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGGCGTCGTACAGCCGGGGG6850.10756554824668156No Hit
GCTCAGCACTGAACACAGAGAACTCACCATGGAGTTTGGACTGAACTGGG6790.1066233682620391No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG6780.1064663382645987No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC6720.10552415827995623No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTTAGCCTGGTACCAGC6710.10536712828251579No Hit
GTCTGGGGGAGGCGTCGTACAGCCGGGGGGGTCCCTGAGACTCTCCTGCG6620.10395385830555212No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG6470.10159840834394596No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6460.10144137834650552No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6450.10128434834906512No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6440.10112731835162471No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT6410.10065622835930348No Hit
GAACTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGC6380.10018513836698224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6700.053.7575071
GGTATCA3450.052.1993141
TCAACGC6200.051.1055874
ATCAACG6400.049.512433
CAACGCA7000.045.2649465
TATCAAC7250.044.704392
CGCAGAG7400.042.8081058
AACGCAG7550.041.957616
CAGCGCT3800.039.783839
GTACATG12750.038.4186971
CTAATAC1502.7543138E-838.4096453
CATGGGA6850.037.845944
TACATGG12800.037.6999242
GGCAGCG4050.037.3309677
ACATGGG13700.035.2205163
GTGCTAT1251.3213246E-534.5768281
CACTAGT850.002829074233.898851
GCAGAGT9700.033.3973249
GTATTAG1301.728276E-533.2469521
CTATAGG1102.52888E-432.743211
Produced by FastQC (version 0.11.5)