FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635862
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAAAGTCCCGGCACTGATTGGGGAAAGGGACGGTGT29010.45623106900553895No Hit
GTAATAGACGGCCGAGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATAGA23460.3689479792785227No Hit
CTATAGAGGTGGCAGTAGTACATATTACGCAGACTCCGTGAAGGGCCGAT18980.29849244018356186No Hit
GTGTGGACGTCTGGGGCCAAGGGACCACGGTCGTCGTCTCCTCAGCCTCC15990.25146965851080894No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15560.2447071848923194No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15020.23621477616212325No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14050.2209598938134375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13500.21231021825490437No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12440.19563993445118594No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA11980.18840566034768552No Hit
ATATTACGCAGACTCCGTGAAGGGCCGATTCACCATAACCAGAGATAATT10950.17220717702897798No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10820.17016270826059743No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10220.16072669856037947No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9750.15333515762854205No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9570.15050435471847665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.14830261912175913No Hit
GGACTGAACAGAGAGGGCTTACCATGGAGTTTGGGCTGAGGTGGCTCTTT9260.1456290830400307No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA9040.14216921281661746No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9020.1418546791599435No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8840.1390238762498781No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT8630.13572127285480184No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8600.13524947236979093No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT8530.13414860457143216No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8460.13304773677307338No Hit
CGTCCACACCGTCCCTTTCCCCAATCAGTGCCGGGACTTTCGCACAGTAA8160.1283297319229644No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8110.1275433977812796No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8000.12581346266957297No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7830.12313992658784453No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7790.12251085927449668No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCT7660.12046639050611611No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7580.11920825587942037No Hit
CCTCTATAGAGAATAGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCG7310.11496205151432229No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7260.11417571737263746No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7240.11386118371596353No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7110.11181671494758297No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC7080.11134491446257208No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7040.1107158471492242No Hit
TCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGC6930.10898591203751759No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.10804231106749577No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6840.10757051058248489No Hit
CTCTTGTGCAGCCTCTGGATTCACCTTTAGTTCATATGCCATGAGTTGGG6830.10741324375414792No Hit
GCCTGGGGGGTCCCTGAGACTCTCTTGTGCAGCCTCTGGATTCACCTTTA6760.10631237595578914No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC6700.10536877498576736No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT6670.10489697450075647No Hit
CCCTGAGAGCCGAGGACTCGGCCGTCTATTACTGTGCGAAAGTCCCGGCA6640.10442517401574554No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6540.10285250573237589No Hit
GAACAGAGAGGGCTTACCATGGAGTTTGGGCTGAGGTGGCTCTTTCTTGT6520.10253797207570196No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6440.10127983744900623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)