FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635862
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAAAGTCCCGGCACTGATTGGGGAAAGGGACGGTGT21480.3378091472678034No Hit
GTAATAGACGGCCGAGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATAGA18260.2871692285433003No Hit
CTATAGAGGTGGCAGTAGTACATATTACGCAGACTCCGTGAAGGGCCGAT14690.23102497082700332No Hit
GTGTGGACGTCTGGGGCCAAGGGACCACGGTCGTCGTCTCCTCAGCCTCC11720.1843167228109244No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11410.17944145113247845No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11000.17299351117066283No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10450.16434383561212967No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10150.1596258307620207No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10120.15915403027700978No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA8920.14028201087657385No Hit
ATATTACGCAGACTCCGTGAAGGGCCGATTCACCATAACCAGAGATAATT8000.12581346266957297No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7760.12203905878948576No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7580.11920825587942037No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6980.1097722461792024No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6950.10930044569419152No Hit
CGTCCACACCGTCCCTTTCCCCAATCAGTGCCGGGACTTTCGCACAGTAA6760.10631237595578914No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6730.10584057547077824No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT6690.10521150815743038No Hit
CCTCTATAGAGAATAGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCG6580.10348157304572375No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6490.10206617159069105No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6400.10065077013565837No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6380.10033623647898444No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT6360.10002170282231049No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6360.10002170282231049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)