FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587364
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTACGAGAGTCCAGACTACGCAGGAGGCTTTTGATACTTG12070.20549437827309813No Hit
GTAATATACAGCCGTGTCGTCTGCTCTAAGGGTGATCATTTCCAGATACA11060.1882989083430377No Hit
CCCTTAGAGCAGACGACACGGCTGTATATTACTGTACGAGAGTCCAGACT9920.16889016010514776No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7890.13432896806750158No Hit
CTATAGGAGAGTAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACC7870.13398846371245088No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7510.12785938532153826No Hit
CTCCTATAGTGGTACTCGGCCTTGGGTGTTCGGCGGAGGGACCAAGCTGA7500.1276891331440129No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT7250.12343282870587916No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6820.11611198507228908No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6630.1128771936993074No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAATCTGGGGGAGGCG6500.11066391539147785No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6030.10266206304778637No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5940.10112979345005824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5350.070.79841
TCAACGC12450.052.395874
ATCAACG12500.052.1862833
TATCAAC12550.051.978372
CAACGCA12900.050.5681085
AACGCAG13150.049.6067356
GTATCAA15100.044.594131
CGTTACA658.630414E-443.1648332
GTACATG20250.042.2589071
TACATGG20600.041.5409132
CGCAGAG16700.039.061598
ACATGGG22000.037.940913
ACAGCGG9100.035.65448145
GCAGAGT18800.033.9521269
GCAGCGC3600.033.123028
CAGCGCT3550.031.613689
CTAGACT900.004264006431.1746064
GGCAGCG3600.031.1746067
CATGGGG14050.029.4550064
TATATAG2004.3403452E-728.0571442
Produced by FastQC (version 0.11.5)