FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839468
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC38120.45409711865133634No Hit
GTCTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC33070.39393997150576315No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAAACGTAGCCG27240.3244912253951312No Hit
GATTATTACTGCTGCTCATATGCAGGCGGCTACGTTTGGGTTTTCGGCGG25170.29983275121862896No Hit
CTCCTGGACAGTCAATCACCATCTCCTGCACTGGAAGCAGCAGTGATGTT24850.2960208131816817No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG23080.27493603091481744No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23040.27445953866019906No Hit
GTCAATAGGCGGCCCTCAGGGGTCCCTGATCGCTTCGCTGGCTCCAAGTC22440.267312154840923No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCA21340.254208617838917No Hit
GTCAATCACCATCTCCTGCACTGGAAGCAGCAGTGATGTTGGTGGTTATA21160.25206440269313424No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20700.24658474176502262No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20050.2388417426274736No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19980.2380078811818914No Hit
CTCCTGCACTGGAAGCAGCAGTGATGTTGGTGGTTATAAGTATGTCTCCT19470.23193260493550677No Hit
GTGTCCGGGTCTCCTGGACAGTCAATCACCATCTCCTGCACTGGAAGCAG18330.21835257567888233No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18070.21525537602386274No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17510.2085844844592051No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17180.2046534233586033No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17080.20346219272205732No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA16880.2010797314489653No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16680.19869727017587327No Hit
GGTTATAAGTATGTCTCCTGGTACCGACAGCACCCAGGCGAAGTCCCCAA16650.1983399009849095No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT16560.19726779341201808No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15930.18976304040177827No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT15340.18273477964615686No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15290.18213916432788385No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAAACGTAGCCGC14840.17677862646342685No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14590.17380054987206184No Hit
TCTCTGGCCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT14250.1697503657078054No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG14230.16951211958049622No Hit
TAGTAATACAGTGGGTTTGTGGGACGAGCTGCCAGAGAACTCGCACAATA14200.16915475038953243No Hit
GTATTACTATGACTTCTGGGGCCAGGGAACCCTGGTCACCGTATCCTCAG14150.1685591350712594No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14150.1685591350712594No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT14010.166891412180095No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13950.16617667379816742No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13590.1618882435066018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.161530874315638No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13000.1548599827509804No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC12550.14949944488652336No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT12400.14771259893170435No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12280.14628312216784917No Hit
GCTCATATGCAGGCGGCTACGTTTGGGTTTTCGGCGGAGGGACCAAGGTG12230.14568750684957615No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC11850.14116083043070135No Hit
GTTATAAGTATGTCTCCTGGTACCGACAGCACCCAGGCGAAGTCCCCAAA11530.13734889239375414No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG11420.13603853869355353No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11400.13580029256624432No Hit
GTCATAATTTATGATGTCAATAGGCGGCCCTCAGGGGTCCCTGATCGCTT11170.13306046210218853No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAATCAC11100.13222660065660632No Hit
TCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGC10950.1304397547017873No Hit
GTATTATTGTGCGAGTTCTCTGGCAGCTCGTCCCACAAACCCACTGTATT10880.1296058932562051No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10870.12948677019255053No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTCTAG10640.1267469397284947No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10430.12424535539174811No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGT10320.1229350016915475No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10200.12150552492769229No Hit
GTATGTCTCCTGGTACCGACAGCACCCAGGCGAAGTCCCCAAAGTCATAA10160.1210290326730739No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC10110.1204334173548009No Hit
CCCGTGGGGGGGGTGAGGGTCTAGAACCTATGAACATTCTGTAGGGGCCA9960.1186465713999819No Hit
CATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGGCCAGAG9860.11745534076343589No Hit
GTCTGGGGCTGAGGTAAAGAAGCCAGGGTCCTCGGTGAAGGTCTCCTGCA9720.1157876178722715No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGG9450.11257129515359728No Hit
CCTCAGGGGTCCCTGATCGCTTCGCTGGCTCCAAGTCTGGCAACACGGCC9360.11149918758070587No Hit
TTATTACTGCTGCTCATATGCAGGCGGCTACGTTTGGGTTTTCGGCGGAG9340.11126094145339667No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG9320.11102269532608747No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9260.11030795694415987No Hit
CAATAATACAGGGCCGTGTCCTCAGATCTCAGGCTGTTCAGCTCCATGTA8790.10470917295239367No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT8780.10459004988873906No Hit
GTATCAACGCAGAGTACATGGGGCTGGGGTCTCAGGAGGCAGCACTCTCG8760.10435180376142986No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAATCACCATCT8730.10399443457046606No Hit
TCCTGGTACCGACAGCACCCAGGCGAAGTCCCCAAAGTCATAATTTATGA8710.10375618844315686No Hit
TTCCAGGACAGAGTCACCATTACCGCGGACCAATCCACGAGCACAGCCTA8670.10327969618853847No Hit
CCTATTGACATCATAAATTATGACTTTGGGGACTTCGCCTGGGTGCTGTC8580.10220758861564706No Hit
ATACAGGGCCGTGTCCTCAGATCTCAGGCTGTTCAGCTCCATGTAGGCTG8530.10161197329737405No Hit
GTACATGGGGCTGGGGTCTCAGGAGGCAGCACTCTCGGGACGTCTCCACC8520.10149285023371944No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG8470.10089723491544646No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8460.10077811185179185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6250.080.757481
GTATCAA19800.073.925721
TCAACGC22450.063.5291864
ATCAACG22600.063.4262543
CAACGCA23400.060.334355
AACGCAG24100.058.543526
TATCAAC24550.058.3883252
ACGCAGA27650.051.2843677
CGCAGAG28050.050.8096548
GTGCTAG2750.044.5739331
GCAGAGT32100.044.399099
GAATACG2500.043.1882069
TGCTAGA4550.031.6623422
CAGAGTA31950.030.8648139
GTACATG25800.029.0655361
GTTAGAC1050.00795529527.4406973
TTATTGA18150.027.3839994
TATTGAG17650.027.3435275
GGTTTAT13100.026.9705311
GTAATAC9200.026.6204573
Produced by FastQC (version 0.11.5)