FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839468
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35570.4237207374194132No Hit
GTCTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC30890.3679711436290603No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAAACGTAGCCG27480.3273501789228416No Hit
GATTATTACTGCTGCTCATATGCAGGCGGCTACGTTTGGGTTTTCGGCGG26140.3113876883931252No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG21920.26111775553088384No Hit
CTCCTGGACAGTCAATCACCATCTCCTGCACTGGAAGCAGCAGTGATGTT21290.25361300252064406No Hit
GTCAATAGGCGGCCCTCAGGGGTCCCTGATCGCTTCGCTGGCTCCAAGTC21040.250634925929279No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21020.25039667980196983No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCA20880.24872895691080543No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20840.24825246465618703No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20460.2437257882373122No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19780.23562541990879937No Hit
GTCAATCACCATCTCCTGCACTGGAAGCAGCAGTGATGTTGGTGGTTATA17920.21346853006904373No Hit
GTGTCCGGGTCTCCTGGACAGTCAATCACCATCTCCTGCACTGGAAGCAG17670.21049045347767875No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17110.2038195619130211No Hit
CTCCTGCACTGGAAGCAGCAGTGATGTTGGTGGTTATAAGTATGTCTCCT16970.2021518390218567No Hit
GGTTATAAGTATGTCTCCTGGTACCGACAGCACCCAGGCGAAGTCCCCAA15930.18976304040177827No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15670.18666584074675865No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15610.18595110236483106No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15510.18475987172828506No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15090.17975670305479186No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT15040.17916108773651884No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15010.17880371854555505No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14460.17225195004455204No Hit
TCTCTGGCCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT14030.1671296583074042No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13990.1666531660527858No Hit
TAGTAATACAGTGGGTTTGTGGGACGAGCTGCCAGAGAACTCGCACAATA13890.16546193541623982No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13820.16462807397065762No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCAAACGTAGCCGC13750.16379421252507542No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13700.1631985972068024No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13520.1610543820610196No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13490.16069701287005578No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13430.15998227448812818No Hit
GTATTACTATGACTTCTGGGGCCAGGGAACCCTGGTCACCGTATCCTCAG13390.1595057822335098No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13160.156765951769454No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12870.15331138292347057No Hit
GCTCATATGCAGGCGGCTACGTTTGGGTTTTCGGCGGAGGGACCAAGGTG12690.1511671677776878No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12510.149022952631905No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG11600.13818275383933634No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC11320.13484730805700754No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT11290.1344899388660437No Hit
GTTATAAGTATGTCTCCTGGTACCGACAGCACCCAGGCGAAGTCCCCAAA11120.13246484678391554No Hit
GTCATAATTTATGATGTCAATAGGCGGCCCTCAGGGGTCCCTGATCGCTT10880.1296058932562051No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10820.12889115487427752No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10760.12817641649234993No Hit
GTATTATTGTGCGAGTTCTCTGGCAGCTCGTCCCACAAACCCACTGTATT10700.1274616781104223No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAATCAC10610.12638957053753092No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10490.12496009377367572No Hit
GTATGTCTCCTGGTACCGACAGCACCCAGGCGAAGTCCCCAAAGTCATAA10220.12174377105500149No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC9660.11507287949034388No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9640.11483463336303468No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9600.11435814110841629No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG9590.11423901804476168No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGT9550.11376252579014329No Hit
TTATTACTGCTGCTCATATGCAGGCGGCTACGTTTGGGTTTTCGGCGGAG9450.11257129515359728No Hit
GTCTGGGGCTGAGGTAAAGAAGCCAGGGTCCTCGGTGAAGGTCTCCTGCA9250.11018883388050527No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTCTAG9010.10732988035279487No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG9000.10721075728914026No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG8920.10625777277990346No Hit
TCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGC8890.10590040358893966No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8610.10256495780661085No Hit
CCTCAGGGGTCCCTGATCGCTTCGCTGGCTCCAAGTCTGGCAACACGGCC8500.10125460410641024No Hit
CATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGGCCAGAG8430.10042074266082804No Hit
TCCTGGTACCGACAGCACCCAGGCGAAGTCCCCAAAGTCATAATTTATGA8400.10006337346986426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13650.068.007961
TCAACGC14350.063.6229674
ATCAACG14700.062.1044243
CAACGCA15050.059.715555
AACGCAG15950.056.3561066
TATCAAC16350.055.8370062
ACGCAGA17300.051.5457847
CGCAGAG17750.050.2389878
GGTATCA5450.050.1752741
GCAGAGT20450.043.2543079
GTCTAAT1502.7750502E-838.3796841
TATTGAG16200.036.8363845
GTACATG24650.035.3241041
GAATACG2302.910383E-1134.393989
TTATTGA17000.034.2529644
GGTTTAT12300.032.7631451
ATTGAGT12400.030.7358916
GATATAC1652.5497793E-630.496523
TTTATTG20600.030.3590553
CATGGGG18600.029.7604734
Produced by FastQC (version 0.11.5)