FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791832
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATGGGTAGTCGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGACCG15080.19044443770900898No Hit
CCCATATAGAGCACACAGTAATAATCAGACTCATCATCTGACTGGGCCCC13870.17516341850291475No Hit
GTAATAATCAGACTCATCATCTGACTGGGCCCCCGTGATGGTGAGGGCAG12140.15331534972064784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11770.14864264136837108No Hit
GATTATTACTGTGTGCTCTATATGGGTAGTCGCATTTCGGTGTTCGGCGG10670.13475080572646722No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9790.12363733721294416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17500.078.3276751
GGTATCA5700.068.346861
ATCAACG19800.067.685863
TCAACGC19850.067.5153664
CAACGCA19950.067.176945
AACGCAG20950.064.2412346
TATCAAC21300.063.257532
ACGCAGA23650.057.2114567
CGCAGAG24750.054.9595158
GCAGAGT34250.039.715279
CCGTCCG1201.0053784E-535.9853949
ACGGTTC950.004899140430.3034928
GAATACG1753.9947827E-628.7883199
TATAGAG11850.027.3617825
AGACCGT2156.526352E-726.7798296
ATAGAGC12500.026.4852526
TATTGAG8700.025.6739485
CCCATAT12550.025.2934821
CAGAGTA28900.024.9033899
CATATAG12000.024.6180043
Produced by FastQC (version 0.11.5)