FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600417
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAGAGCCGTGTCCTCGGCTCTGAGGCCATTCATTTCCAGAAACA29930.49848688494829424No Hit
CTCTATTACTGTGCAAGGGATTGGCACGATATGGCGGGTGGTCATTTTGA21700.36141548290604697No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10710.17837602866008123No Hit
CATTAGTAGCAGTGGTTTTTCCATTTTCTACGCAGACTCTGTGAAGGGCC10070.16771677017805958No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9810.1633864464197383No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9110.15172788245502708No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8740.14556549864510832No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCGACTGTGAGGTGC8630.14373343859351084No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAAAATCCTCGAACCACTC8510.14173482762813178No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8400.1399027675765343No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8170.1360720965595578No Hit
CTCCTGTACTGGGAGCAACTCCAACATCGGGGCAAATTTTGGTGTACACT8040.13390693468039713No Hit
CTCCAGAGACAATGCCAGGAATTCACTGTTTCTGGAAATGAATGGCCTCA7890.1314086709736733No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA7790.1297431618358574No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC7520.12524628716375452No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA7510.12507973624997293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.12174871797434117No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC6990.11641908873333033No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6860.11425392685416969No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6840.11392082502660651No Hit
CTATTACTGTGCAAGGGATTGGCACGATATGGCGGGTGGTCATTTTGACT6760.11258841771635379No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA6700.11158911223366427No Hit
CAGTAATAGAGAGCCGTGTCCTCGGCTCTGAGGCCATTCATTTCCAGAAA6340.1055932793375271No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT6070.10109640466542419No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12300.063.9622151
GGTATCA6200.061.700161
ATCAACG12750.059.8515173
TCAACGC12750.059.8515174
CAACGCA13250.057.5929765
AACGCAG13950.054.7030036
ACGATAG400.006220618753.9934125
TATCAAC14450.052.8497772
GAATACG1751.8189894E-1245.251629
CGCAGAG16750.045.1288228
GCAGAGT18350.041.1938829
GTAATAG13850.038.564181
TAGAGAG14100.037.272055
AATAGAG14400.035.9956053
TAATAGA14550.035.651242
AGAGAGC16350.034.3444336
GTACATG13700.033.191131
ATAGAGA15900.033.052574
GAGAGCC16500.032.7232787
CTCTATT13250.032.6841741
Produced by FastQC (version 0.11.5)