FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807908
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATAGCTGTGTCCTCAATTGTCAGGCCGCTCATCTGCAGAAACA71550.39576128873814376No Hit
ATCTATTACTGTGCGAGTGGCTTTAGTAATGGCCATAACTACTTTGACAA62720.34692030789177325No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.13800480997926884No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22030.12185354564502177No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20290.11222916210338137No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20130.11134416131794317No Hit
TACCTATGCTATGCATTGGGTCCGCCAGGCTCCCGGCAAGGGGCTGGAGT18980.10498321817260611No Hit
ACCAAATACTACGCAGACTCCGTGCAGGGCCGATTCACCATCTCCAGAGA18640.10310259150354995No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18250.10094540208904436No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18150.1003922765981455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC37500.064.708724
ATCAACG38000.063.857293
CAACGCA39150.061.9815335
AACGCAG40500.060.091616
GTATCAA43200.058.363421
CGCAGAG47750.050.8141568
TATCAAC52250.047.544142
GCAGAGT56800.042.7178849
GGTATCA24400.038.6756741
TAGATAG30800.036.703985
GTAATAG31600.035.1067051
GATAGCT33600.033.2139557
TAATAGA32900.033.0480042
AATAGAT33800.032.5940933
CTGTGCG30250.032.3699269
ATAGATA35250.032.2746964
ACGCAGA75800.032.105227
GTACATG51700.031.3507841
GTATACG1652.510118E-630.5612091
ACATGGG51050.029.902283
Produced by FastQC (version 0.11.5)