FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116945
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTAGG27300.24441669016827147No Hit
GCCTACATGGACCTGAGGAGCCTGACATCTGACGACACGGCCGTGTATTA25620.22937566308099325No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCA22640.20269574598570209No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19040.1704649736558201No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18980.16992779411698877No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17700.15846796395525295No Hit
GTGTATTATTGTGCGAGAGATTTGGGATGGAATGACTACGAGTCGGTGTC17470.15640877572306602No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG17410.15587159618423466No Hit
GTATTATTGTGCGAGAGATTTGGGATGGAATGACTACGAGTCGGTGTCGG17250.15443911741401772No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17000.15220086933555366No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16920.15148462995044518No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA16650.14906732202570402No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC16190.14494894556133023No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG16160.14468035579191454No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16160.14468035579191454No Hit
CAATAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTA15590.13957715017301658No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15550.13921903048046233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.13554830363178133No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14990.1342053547847029No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14980.13411582486156434No Hit
GTATCAACGCAGAGTACATGGGACCCAAAAACCACACCCCTCCTTGGGAG14910.13348911539959443No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14790.1324147563219317No Hit
CATGTAGGCTGTGTCCGTGGACGTGTCTGTGGTCATGGTGACTCTGTCTT14570.13044509801288334No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA14500.12981838855091343No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC14130.1265057813947867No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14080.12605813177909386No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG13290.11898526785114755No Hit
ACTATGGTCTGAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGG13230.11844808831231618No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12950.11594125046443648No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12240.10958462592159865No Hit
TCCACGGACACAGCCTACATGGACCTGAGGAGCCTGACATCTGACGACAC12140.10868932669021304No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12110.10842073692079734No Hit
ATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTAGGCTG11920.10671966838116469No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11840.10600342899605622No Hit
GACCTGGAGCATCCTTTTCTTGGCGGCAGCAGCAACAGGTGCCCGCTCCC11440.10242223207051378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9200.074.50411
GTATCAA22850.072.309221
TCAACGC26650.059.738984
ATCAACG27150.058.904153
CAACGCA27100.058.7475
TATCAAC29000.055.394872
AACGCAG29200.054.473236
ACGCAGA32100.049.549757
CGCAGAG33000.048.1983958
GAATACG2500.046.0610929
GCAGAGT37700.042.1895759
GTACATG31200.033.7631231
TACGGGT1000.006267384628.8152714
GTTCCGA1555.7809462E-527.8607796
GTGCTAG5200.027.7505131
ATAGGCG1050.007952983527.4431155
CATGGGG15300.027.3085924
CAGAGTA39700.025.9238669
TCTACAC7100.025.3655573
CCTTATA4101.8189894E-1224.6370411
Produced by FastQC (version 0.11.5)