FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116945
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTAGG25050.22427245746209526No Hit
GCCTACATGGACCTGAGGAGCCTGACATCTGACGACACGGCCGTGTATTA23220.2078884815277386No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCA21860.19571241198089434No Hit
GTATTATTGTGCGAGAGATTTGGGATGGAATGACTACGAGTCGGTGTCGG19030.17037544373268157No Hit
GTGTATTATTGTGCGAGAGATTTGGGATGGAATGACTACGAGTCGGTGTC17210.15408099772146344No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17070.1528275787975236No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16730.14978356141081253No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15340.13733890209455255No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15300.13698078240199832No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG15270.1367121926325826No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA15260.13662266270944406No Hit
CAATAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTA15070.1349215941698114No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15040.13465300440039574No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14900.13339958547645586No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG14690.1315194570905461No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA14520.12999744839719055No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13560.1214025757758887No Hit
GTATCAACGCAGAGTACATGGGACCCAAAAACCACACCCCTCCTTGGGAG13420.12014915685194885No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA13330.11934338754370179No Hit
CATGTAGGCTGTGTCCGTGGACGTGTCTGTGGTCATGGTGACTCTGTCTT13150.1177318489272077No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13020.11656795992640641No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG12900.11549360084874367No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12750.11415065200166526No Hit
ACTATGGTCTGAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGG12400.11101710469181562No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11380.10188505253168241No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC11280.1009897533002968No Hit
ATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTAGGCTG11250.1007211635308811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16000.059.306444
GTATCAA16800.057.8698161
CAACGCA16700.056.8307275
ATCAACG16950.056.4040573
AACGCAG17900.053.424926
GGTATCA7100.051.7296371
TATCAAC19050.050.943252
ACGCAGA21400.044.3512087
CGCAGAG22650.041.9035648
GCAGAGT26850.035.0810249
GAATACG3600.033.954069
GTGCTAG5150.033.5607451
TCTACAC7550.026.658733
GTACATG30100.024.6432691
GTATTAG4600.023.4834021
ATTAGAG5050.022.7749253
CAGAGTA27100.022.2871869
CAGTGCG1952.7655164E-422.123919
CTATAGG2353.906428E-521.4515061
AGAGTAC22050.020.5427115
Produced by FastQC (version 0.11.5)