FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822661
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA57280.6962770813251145No Hit
GTATATTACTGTGCGAAAGATCTCCGATATTGTAGTAGTACCAGCTGCCG33420.4062426686083332No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG23100.2807960994869089No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21290.25879432718944984No Hit
GCCGTATATTACTGTGCGAAAGATCTCCGATATTGTAGTAGTACCAGCTG17780.21612790687780264No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17450.21211653402798966No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17220.20932072870842303No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17160.20859138819027523No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17160.20859138819027523No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15740.19133032926077692No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC15430.1875620699170132No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA15040.1828213565490524No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG14940.18160578901880606No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT14670.17832375668714087No Hit
GTCTACAAGATTACAATTACCCTCGGACCTTCGGCCAAGGGACCAAGGTG14540.1767435188978206No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC14420.175284837861525No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA14320.1740692703312786No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14310.17394771357825398No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13860.16847765969214537No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13790.1676267624209729No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13790.1676267624209729No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGG13530.1644662868423324No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12510.15206749803381953No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG12410.15085193050357315No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12320.14975791972635144No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12320.14975791972635144No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC12300.14951480622030217No Hit
CTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATG12200.14829923869005582No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12010.14598966038258773No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11900.14465253609931675No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11610.1411273902616023No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11530.1401549362374052No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT11470.1394255957192574No Hit
GACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTC11400.13857469844808493No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11130.13529266611641977No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11050.13432021209222267No Hit
ACTTATTACTGTCTACAAGATTACAATTACCCTCGGACCTTCGGCCAAGG11030.1340770985861734No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10850.13188907703172997No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10790.13115973651358215No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10690.1299441689833358No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAAA10620.12909327171216334No Hit
GTGTGAGGTGCAGGTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG10460.12714836366376916No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10260.12471722860327644No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10230.12435255834420253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10220.1242310015911779No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA10170.12362321782605473No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA10030.1219214232837098No Hit
ATATTACTGTGCGAAAGATCTCCGATATTGTAGTAGTACCAGCTGCCGTA9960.12107052601253736No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9870.11997651523531563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9630.11705915316272439No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9420.11450646134920701No Hit
GTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCTTCCC9400.11426334784315774No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9400.11426334784315774No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG9350.11365556407803457No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT9310.11316933706593602No Hit
GATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCG9240.11231843979476357No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA9100.11061664525241867No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGGTGTTGGAGTCTGGGGGAGGCT9050.1100088614872955No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG8950.10879329395704913No Hit
AGCACAGACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA8870.10782083993285205No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8460.10283701305884199No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA8450.10271545630581734No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8440.10259389955279272No Hit
TGCAGATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAACCGGCC8410.10222922929371879No Hit
GTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATATTACT8410.10222922929371879No Hit
GTACTACTACAATATCGGAGATCTTTCGCACAGTAATATACGGCCGTGTC8360.10162144552859562No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8360.10162144552859562No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8310.10101366176347244No Hit
GTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG8290.10077054825742317No Hit
CTGTAGCAGCCACAGAGGTCACGGTCAGGTGGTCCTTGAACAGCGCTCTT8270.10052743475137389No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)