FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822661
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA47230.5741125445353554No Hit
GTATATTACTGTGCGAAAGATCTCCGATATTGTAGTAGTACCAGCTGCCG33020.40138039848734774No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG17840.21685724739595047No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17790.21624946363082725No Hit
GCCGTATATTACTGTGCGAAAGATCTCCGATATTGTAGTAGTACCAGCTG17120.20810516117817668No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14060.1709087947526381No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13990.17005789748146563No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13600.16531718411350482No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA13480.1638585030772092No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13400.1628860490530121No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12850.15620042763665715No Hit
GTCTACAAGATTACAATTACCCTCGGACCTTCGGCCAAGGGACCAAGGTG12680.15413396283523834No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12510.15206749803381953No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11930.14501720635839063No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11810.143558525322095No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11620.14124894701462692No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11550.14039804974345446No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGG11430.13893936870715884No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT11350.13796691468296174No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11180.13590044988154296No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11060.1344417688452473No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10530.12799926093494163No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10490.12751303392284308No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG10330.1255681258744489No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10230.12435255834420253No Hit
CTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATG10230.12435255834420253No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC10220.1242310015911779No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10040.12204298003673446No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9960.12107052601253736No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9730.11827472069297074No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9550.11608669913852729No Hit
ACTTATTACTGTCTACAAGATTACAATTACCCTCGGACCTTCGGCCAAGG9510.11560047212642874No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9490.11535735862037946No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9470.11511424511433019No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9380.11402023433710846No Hit
ATATTACTGTGCGAAAGATCTCCGATATTGTAGTAGTACCAGCTGCCGTA9270.11268311005383749No Hit
GTGTGAGGTGCAGGTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG9200.11183221278266503No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA9140.11110287226451722No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG9110.1107382020054433No Hit
GACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTC9040.10988730473427086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8960.10891485071007377No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8940.10867173720402451No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC8440.10259389955279272No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8360.10162144552859562No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8260.10040587799834927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9700.054.176354
ATCAACG9800.052.888963
GTATCAA10150.051.8185351
TATCAAC11050.047.557522
CAACGCA11550.045.4987535
GAATACG2350.042.8863229
AACGCAG12750.041.7811286
TACGGCC22750.036.7058457
CGCAGAG14100.036.249158
TATACGG22850.035.9151155
ATACGGC22950.035.444956
GTGCTAG3050.035.4339641
TACATGG20700.034.776692
GTACATG21600.034.690291
AATATAC23800.034.179063
TAATATA24500.033.496342
TCTACAC5300.032.598233
GTAATAT25400.032.053321
ATATACG26800.030.8902664
GCAGAGT16800.029.9948989
Produced by FastQC (version 0.11.5)