FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581772
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTGGTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCA33790.5808117269308252No Hit
CTATAATACTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT23060.39637521228247496No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21810.3748891318248386No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16440.28258493017883296No Hit
AGCACATACTATGCAGACTCCGTGAGGGGCCGGTTCAGCATCTCCAGAGA16010.27519371850140606No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12560.2158921364383298No Hit
TTATTATTGTCTGCAGTATTATAGTGGTCCTCAGACGTTCGGCCAAGGGA12510.21503269322002433No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11310.19440605598069347No Hit
GTATGTGCTAGTAGCAGTGGCACTAAAAGCTGAGATCCACTCCAGTCCCT11290.19406227869337128No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10940.18804617616523311No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10560.18151440770611166No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10430.17927985533851748No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10300.1770453029709233No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10050.17274808687939605No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9840.16913842536251314No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9710.16690387299491896No Hit
ATACTATGCAGACTCCGTGAGGGGCCGGTTCAGCATCTCCAGAGACAATT9580.16466932062732478No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9460.1626066569033917No Hit
ATAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAACA9120.15676244301891462No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8880.15263711557104845No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8750.15040256320345427No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8690.14937123134148772No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8460.14541779253728265No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8090.1390579127218223No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGACAACCTCCTAAATTGCTCATT8060.13854224679083904No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8020.13785469221619467No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7540.1296040373204623No Hit
CTATTATTGTGCATCCCCACTCGATTGTAGTGGGGCCACCTGCTCTTCCT7440.12788515088385138No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7280.12513493258527397No Hit
CTATGCAGACTCCGTGAGGGGCCGGTTCAGCATCTCCAGAGACAATTCCA7280.12513493258527397No Hit
GTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTG7230.12427548936696851No Hit
GTCTCCAGCCTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT7180.12341604614866306No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA7130.12255660293035761No Hit
CTCCTAAATTGCTCATTTTCTGGGCATCTATTCGGGAATCCGGGGTCCCT7110.12221282564303543No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6840.11757183226418597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6820.11722805497686378No Hit
GTTTATTATTGTCTGCAGTATTATAGTGGTCCTCAGACGTTCGGCCAAGG6750.11602483447123615No Hit
TCTGAGGACCACTATAATACTGCAGACAATAATAAACTGCCACATCTTCA6750.11602483447123615No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6700.1151653912529307No Hit
CCCCATGGGCTGGGCCCGCCAGACTCCAGAGAAGGGACTGGAGTGGATCT6620.11379028210364198No Hit
TTATAGTGGTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGC6610.11361839345998088No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6570.11293083888533652No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC6540.11241517295435324No Hit
GACCAGGACAACCTCCTAAATTGCTCATTTTCTGGGCATCTATTCGGGAA6460.11104006380506452No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT6460.11104006380506452No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6440.11069628651774234No Hit
GTCTATTATTGTGCATCCCCACTCGATTGTAGTGGGGCCACCTGCTCTTC6400.11000873194309797No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6320.10863362279380927No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6290.10811795686282599No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6250.10743040228818164No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG6140.10553962720790963No Hit
GCATAGTATGTGCTAGTAGCAGTGGCACTAAAAGCTGAGATCCACTCCAG6110.10502396127692636No Hit
CAATAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAA6060.10416451805862091No Hit
GTACCAGCAGAGACCAGGACAACCTCCTAAATTGCTCATTTTCTGGGCAT6050.10399262941495982No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTGTTT6010.10330507484031545No Hit
ATTGTAGTGGGGCCACCTGCTCTTCCTACTGGGGCCGGGGAACCCTGGTC5920.10175807704736564No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC5870.10089863382906018No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA5840.10038296789807691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)