FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581772
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTGGTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCA39870.6853200222767682No Hit
CTATAATACTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT31060.5338861272113474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20070.3449805078278088No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15150.26041129514655226No Hit
TTATTATTGTCTGCAGTATTATAGTGGTCCTCAGACGTTCGGCCAAGGGA14110.24253487620579883No Hit
AGCACATACTATGCAGACTCCGTGAGGGGCCGGTTCAGCATCTCCAGAGA13770.23669066232132174No Hit
GTATGTGCTAGTAGCAGTGGCACTAAAAGCTGAGATCCACTCCAGTCCCT11500.1976719402102542No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11310.19440605598069347No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10920.18770239887791093No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10520.18082685313146732No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10280.17670152568360112No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9790.1682789821442077No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9630.16552876384563026No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9590.1648412092709859No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9270.15934077267383098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8960.15401222472033718No Hit
ATACTATGCAGACTCCGTGAGGGGCCGGTTCAGCATCTCCAGAGACAATT8730.1500587859161321No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8640.14851178812318228No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8220.14129246508941648No Hit
TCTGAGGACCACTATAATACTGCAGACAATAATAAACTGCCACATCTTCA8130.13974546729646667No Hit
GTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTG7890.1356201398486005No Hit
GTTTATTATTGTCTGCAGTATTATAGTGGTCCTCAGACGTTCGGCCAAGG7860.1351044739176172No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7730.13286992155002303No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGGGTGA7680.13201047833171758No Hit
ATAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGAAACA7590.13046348053876777No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGGGT7520.12926026003314012No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7470.12840081681483467No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA7240.1244473780106296No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7230.12427548936696851No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7120.12238471428669652No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGACAACCTCCTAAATTGCTCATT7050.12118149378106888No Hit
GTCTATTATTGTGCATCCCCACTCGATTGTAGTGGGGCCACCTGCTCTTC7050.12118149378106888No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7030.1208377164937467No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA7020.1206658278500856No Hit
TTATAGTGGTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGC7000.12032205056276342No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6840.11757183226418597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6770.11636861175855834No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6660.11447783667828633No Hit
CCCCATGGGCTGGGCCCGCCAGACTCCAGAGAAGGGACTGGAGTGGATCT6660.11447783667828633No Hit
CTATTATTGTGCATCCCCACTCGATTGTAGTGGGGCCACCTGCTCTTCCT6580.11310272752899761No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6510.11189950702336997No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6480.1113838410923867No Hit
CCACTATAATACTGCAGACAATAATAAACTGCCACATCTTCAGCCTGCAG6400.11000873194309797No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT6370.1094930660121147No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6320.10863362279380927No Hit
CTATGCAGACTCCGTGAGGGGCCGGTTCAGCATCTCCAGAGACAATTCCA6160.10588340449523181No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT6020.10347696348397654No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC5970.10261752026567109No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTGTTT5950.1022737429783489No Hit
GACCAGGACAACCTCCTAAATTGCTCATTTTCTGGGCATCTATTCGGGAA5930.10192996569102673No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT5930.10192996569102673No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACCCTCAC5910.10158618840370455No Hit
CTCCTAAATTGCTCATTTTCTGGGCATCTATTCGGGAATCCGGGGTCCCT5860.10072674518539909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3250.077.501141
GTATCAA8100.074.630721
TCAACGC10850.056.9630854
CAACGCA11150.056.0894745
ATCAACG11000.055.532993
AACGCAG11950.052.357076
TATCAAC11800.051.7680442
CGCAGAG13200.046.85428
GCAGAGT14950.039.9264689
ACGCAGA15850.039.0205357
ATACTGC14550.035.092916
ATAATAC14750.034.1060033
TACTGCA15050.033.927037
ATTATAG17400.033.867993
GCGCCTT850.002851951933.8427059
TATAATA16100.033.0316772
GTATTAT17250.032.958041
TAATACT15450.032.560754
CAGAGTA14800.032.556459
CTATAAT15400.032.2442131
Produced by FastQC (version 0.11.5)