FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274464
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC54570.4281800035152033No Hit
ACTATATACTCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG43990.34516471238104807No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAGAGTGCTGCCA34520.27085896502372764No Hit
CTTCTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCC34320.26928967785673036No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT33270.2610509202299947No Hit
GTAATAAATGGCCGTGTCGTCAGAGGTCAGCCTGCTCAACTCCATGTAGG31320.24575037035177139No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29240.2294297838149999No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC28690.2251142441057574No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTTTGTCTCCT26340.20667511989353954No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26160.20526276144324204No Hit
ATATAGAAGTGGCAGCACTCTCGTATTCGGCGGAGGGACCAAGCTGACCG26080.2046350465764431No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24830.19482700178271023No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT24440.19176689180706555No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG24440.19176689180706555No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23610.1852543500640269No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAG23390.18352813418032993No Hit
ATTTATTACTGTGCGAGAGATGGTAAAGCGACTACAGTAACTACGAGCGG23170.18180191829663292No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC23110.18133113214653376No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT22930.17991877369623624No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG22700.17811409345418935No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA22330.17521091219524443No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21350.16752140507695784No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAGAGTGCTGCCAC21060.16524593868481183No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA20900.163990508951214No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20880.16383358023451428No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20220.1586549325834233No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA19960.15661485926632684No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA19840.1556732869661285No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19650.1541824641574811No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19620.15394707108243152No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT19440.15253471263213397No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT19220.150808496748437No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT19070.14963153137318905No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT18900.14829763728124137No Hit
GATTATTACTGCAGTTCATATAGAAGTGGCAGCACTCTCGTATTCGGCGG18640.1462575639641449No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18260.14327591834685013No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18130.14225588168830192No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18080.14186355989655258No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17870.14021580837120545No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT17640.13841112812915862No Hit
GCCTACATGGAGTTGAGCAGGCTGACCTCTGACGACACGGCCATTTATTA17150.1345663745700153No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17040.1337032666281668No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG16940.1329186230446682No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT16790.13174165766942025No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16000.12554297335978104No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15120.1186381098249931No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15050.11808885931654406No Hit
CTCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGC14960.11738268009139528No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATA14840.11644110779119694No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14700.11534260677429885No Hit
GCTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA14590.11447949883245034No Hit
CTATATACTCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG14530.11400871268235117No Hit
GTATATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTC14320.11236096115700406No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14200.11141938885680569No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14200.11141938885680569No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAACAGGGCGCCAGGG14160.11110553142340623No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT14140.11094860270670649No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13790.1082023501644613No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG13710.10757463529766238No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC13680.1073392422226128No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGC13570.1064761342807643No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13410.10522070454716649No Hit
GTTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG13280.10420066788861827No Hit
GAATACGAGAGTGCTGCCACTTCTATATGAACTGCAGTAATAATCAGCCT13080.10263138072162102No Hit
TCCTGGAACAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT13060.10247445200492128No Hit
CCGTAGAGACCGCTCGTAGTTACTGTAGTCGCTTTACCATCTCTCGCACA13030.1022390589298717No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT12930.10145441534637308No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTT12900.10121902227132348No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG12870.10098362919627388No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG12860.10090516483792403No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC12760.10012052125442539No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)