FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274464
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC47620.3736472744620484No Hit
ACTATATACTCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG37230.29212280613654057No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT29170.22888053330655084No Hit
CTTCTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCC29010.22762510357295304No Hit
GTAATAAATGGCCGTGTCGTCAGAGGTCAGCCTGCTCAACTCCATGTAGG28450.2232310995053607No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAGAGTGCTGCCA28240.22158334798001358No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC27890.21883709543776836No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25650.201261079167399No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24870.19514085921610969No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAG23400.1836065985386798No Hit
ATATAGAAGTGGCAGCACTCTCGTATTCGGCGGAGGGACCAAGCTGACCG23180.1818803826549828No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTTTGTCTCCT22140.17372008938659703No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21720.17042458633590277No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT20700.16242122178421672No Hit
ATTTATTACTGTGCGAGAGATGGTAAAGCGACTACAGTAACTACGAGCGG20340.15959650488362168No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT20130.15794875335827455No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG19910.15622253747457754No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19850.15575175132447838No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG18850.14790531548949207No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18810.1475914580560926No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18400.14437441936374823No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA18100.14202048861325234No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA17910.1405296658046049No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT17430.1367633766038115No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17240.1352725537951641No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17220.13511562507846436No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA17190.13488023200341476No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGAGAGTGCTGCCAC17120.13433098149496572No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17080.1340171240615663No Hit
GATTATTACTGCAGTTCATATAGAAGTGGCAGCACTCTCGTATTCGGCGG16790.13174165766942025No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA16210.1271907248851282No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC15990.1254645090014312No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15970.12530758028473146No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15910.12483679413463229No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT15850.12436600798453311No Hit
GCCTACATGGAGTTGAGCAGGCTGACCTCTGACGACACGGCCATTTATTA15830.12420907926783337No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT15610.12248286338413639No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15530.12185514851733747No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15350.12044279006703995No Hit
GTATATAGTAGCCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTC15330.12028586135034022No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15160.11895196725839254No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15120.1186381098249931No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14810.11620571471614732No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT14300.11220403244030432No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG13960.10953624425640897No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATA13720.10765309965601225No Hit
CTCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGC13360.10482838275541718No Hit
GTTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG13190.1034944886634695No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13060.10247445200492128No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12790.10035591432947498No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)