FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552444
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTATTGTGTGAGAGTCGGCATACCTGGAACCCGAGGACTTGCCTA33680.2169482441878741No Hit
ATAATAGACAGCCGTGTCGTCGGCCCCTAGGCTGTTCATCTGCAGATACA31770.20464506288149523No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA28890.18609366907920674No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT28670.1846765487193097No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT23580.15188953675623726No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23240.1496994416545782No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA22550.14525483688944657No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21550.13881338070809637No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT19720.12702551589622554No Hit
GCCTAGGGGCCGACGACACGGCTGTCTATTATTGTGTGAGAGTCGGCATA19210.12374037324373696No Hit
GTACACAATATCCATTCACTATTGGCCAGGGGACCAAGCTGGAGATCGAA18340.11813630636596231No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA18230.11742774618601379No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG17170.11059980263378259No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17160.11053538807196911No Hit
CTATTATTGTGTGAGAGTCGGCATACCTGGAACCCGAGGACTTGCCTACT16970.10931151139751258No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT16710.10763673279036153No Hit
CTCCAGAGACAATGCCAGGAATTCACTGTATCTGCAGATGAACAGCCTAG16040.10332095714885689No Hit
CATTACGGGGACGCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGC15620.10061554555268983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21500.047.209134
ATCAACG21750.045.673593
CAACGCA23300.042.635225
TATCAAC23900.042.167272
GGTATCA12000.040.8365331
GTATCAA25950.040.544941
AACGCAG25450.039.3162736
CGCAGAG26200.037.9160548
GAATACG5800.033.5105179
GTGCTAG6750.033.0962751
GTACACG13150.031.7502825
TACATGG35500.031.6330872
GTACATG35900.031.515661
ACATGGG34500.030.6721063
TACACGG13750.029.8412866
TCCGCTA2901.2732926E-1129.7871258
GCAGAGT33700.029.4777649
TCTACAC18400.028.5594863
ACACGGG14550.028.2005277
ATACCGT2105.309812E-727.4230696
Produced by FastQC (version 0.11.5)