FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523679
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC52590.3451514393779792No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36440.2391579853761849No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTG35960.23600771553588384No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG35470.23279181507390992No Hit
ATAGTATACGGCCGTGTCGTCGACTCTCAGAGTGTTCATTTGCAGATACA33090.21717172711575075No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG31180.20463627837621964No Hit
GTATACGGCCGTGTCGTCGACTCTCAGAGTGTTCATTTGCAGATACAGCG29420.19308528896178262No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28800.1890161904180605No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA28310.18580028995608655No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27730.18199371389905616No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT27030.17739957038195053No Hit
GCCGTATACTATTGTGCAAGAGATTTGAGGGGATCCAACCACTGGGGCCA26580.17444619240666834No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG26090.17123029194469438No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT25580.16788313023937457No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25360.16643925656256991No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA24690.16204200491048312No Hit
GATTATTACTGCAGCTCATCTACAAACAGGAACACTTGGGTGTTCGGCGG24320.15961367190858441No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24230.15902299631352798No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGGCCCCAA24210.15889173507018212No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23990.15744786139337746No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG23320.15305060974129064No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22980.15081916860441077No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC22610.14839083560251207No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGGGACGTT22580.14819394373749326No Hit
CTCCTGCACTGGAACCAGCAGGGACGTTGGTGGTTATAACTATGTCTCCT22350.14668443943901568No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTGT22350.14668443943901568No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT22040.14464989016715463No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21780.14294349400365824No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCC21030.13802119737818794No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20900.13716799929643975No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20310.1332957926177364No Hit
GTATACTATTGTGCAAGAGATTTGAGGGGATCCAACCACTGGGGCCAGGG19960.1309987208591836No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19850.1302767840207813No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTG19760.12968610842572484No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT19480.1278484510188826No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19220.1261420548553862No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA18190.11938210082307363No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT17970.11793822714626899No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17690.11610056973942674No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17560.11524737165767855No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17560.11524737165767855No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA17360.11393475922421979No Hit
GGTATCAACAACACCCAGGCAAAGGCCCCAAACTCATGATTTATGATGTC16900.11091575062726466No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG16790.11019381378886235No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16770.11006255254551647No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16750.10993129130217061No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT16620.10907809322042242No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16580.10881557073373065No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16140.1059278233801214No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC16010.10507462529837322No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.10356512099989565No Hit
GATTAAGACTGATGGGAGTGTGACAACCTACGCGGACTCCGTGAAGGGCC15450.1013993104846887No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGGGACGTTGGTGGTTATA15340.10067737364628639No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG30800.052.8262943
TCAACGC31100.052.316714
CAACGCA32050.050.9906125
AACGCAG33600.048.852636
ACGCAGA37500.043.3879937
CGCAGAG38850.041.8803068
CAGAGCG15300.041.8784879
ATAGGTG16300.037.5425454
TAGGTGG16350.036.547095
GCAGAGT44350.036.524259
GTCATAG16900.036.2954331
GAATACG5600.035.9966749
GTGGTCA18150.032.922588
GTGCTAG6750.032.0728261
ACAGAGC21700.031.1860628
TAGACGA950.004893038430.3129925
TATTGAG18000.029.5972675
CTAGAGA8100.029.3306264
AGGTGGT21850.028.9950336
TCTACAC13700.028.9024433
Produced by FastQC (version 0.11.5)