FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621496
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA28900.4650070153307503No Hit
ATATTATACTATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA27210.43781456356919435No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26390.4246205928919896No Hit
GTAGTACACAGCCGTGTCCTCAGCTCGCAGGCTGTTCATTTGCAGATACA24250.390187547466114No Hit
ATACTATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA21350.34352594385160967No Hit
GTGTACTACTGTGCGAGAGATCCCCACGACACAATTCTAGGCTTGATTGA21180.34079060846731113No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20120.3237349878358027No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19960.32116055453293346No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19300.31054101715859794No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG17060.2744989509184291No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16220.26098317607836574No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16130.2595350573455018No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT16110.2592132531826432No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC14870.23926139508540684No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT14030.22574562024534348No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13720.2207576557210344No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13160.21174713916099216No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12950.20836819545097637No Hit
GTATAATATCGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT12410.1996794830537928No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11170.17972762495655645No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10760.1731306396179541No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG10610.17071710839651422No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10360.16669455636078107No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG10320.1660509480350638No Hit
GAATAGTATAATATCGCTGACAGTAATAAACTGCCACATCTTCAGCCTGC10150.16331561265076525No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9960.16025847310360805No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9400.15124795654356585No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9240.14867352324069663No Hit
GCCATACACTGGGTCCGTCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC9060.1457772857749688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.1395021045992251No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8490.13660586713349726No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT8300.13354872758634004No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT8260.13290511926062276No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8090.13016978387632425No Hit
TTATTACTGTCAGCGATATTATACTATTCCGTACACTTTTGGCCAGGGGA7690.12373370061915122No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA7640.12292919021200457No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC7460.12003295274627672No Hit
GTTTATTACTGTCAGCGATATTATACTATTCCGTACACTTTTGGCCAGGG7290.1172976173619782No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7290.1172976173619782No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7200.11584949862911427No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA7070.11375777157053303No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT7060.1135968694891037No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT7000.11263145700052776No Hit
ATATTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACT6990.11247055491909842No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA6920.11134424034909315No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC6840.11005702369765855No Hit
GTACTACTGTGCGAGAGATCCCCACGACACAATTCTAGGCTTGATTGACT6780.10909161120908259No Hit
ATTCTAGGCTTGATTGACTACTGGGGCAAGGGAACCCTGGTCACCGTCTC6700.10780439455764801No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC6680.10748259039478934No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6630.10667807998764273No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC6540.10522996125477879No Hit
GTACATGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCA6530.10506905917334947No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT6530.10506905917334947No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6460.10394274460334418No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA6390.1028164300333389No Hit
GCTGTGTACTACTGTGCGAGAGATCCCCACGACACAATTCTAGGCTTGAT6340.10201191962619229No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTTGCCCCAGTAGTCAATCAAGC6320.10169011546333362No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6270.100885605056187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)