FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085837
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTACTGTGCCAGAGGCGCGTGGCAAGGGGTTTCGGGGTTCGCCCC50480.4648948230719712No Hit
GTAATATATGGCCGTGTCCGCGGCGGTCACAGACTTCAGTTTCAGGGAGA41250.3798912728153489No Hit
GCCATATATTACTGTGCCAGAGGCGCGTGGCAAGGGGTTTCGGGGTTCGC29750.2739821906971304No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC28260.2602600574487699No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA26530.24432764770402926No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22590.20804227522178745No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC22270.20509523989328046No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGACATTGGTGTTA21320.19634622876177546No Hit
TCTCTGGACTAAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT21010.19349128828728437No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA20680.19045215810476157No Hit
ATATATGGCCGTGTCCGCGGCGGTCACAGACTTCAGTTTCAGGGAGAACT20570.1894391147105873No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG20500.18879445073247642No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAG20450.18833397646239722No Hit
ATATTACTGTGCCAGAGGCGCGTGGCAAGGGGTTTCGGGGTTCGCCCCCT20230.18630788967404868No Hit
GTCTTATGATAACACCAATGTCGTTTTCGGCGGAGGGACCAAGCTGACCG20060.18474227715577937No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTTAGTCCAGA19420.17884820649876546No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19240.1771904991264803No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC19150.17636164544033772No Hit
TCTTTAGACACTTCCAAGAATCAGTTCTCCCTGAAACTGAAGTCTGTGAC18840.1735067049658466No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18600.1712964284694664No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18320.16871777255702283No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA18300.16853358284899114No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17900.16484978868835748No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17880.16466559898032576No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGAC17480.1609818048196921No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17480.1609818048196921No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17430.16052133054961287No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC17100.1574822003670901No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16770.1544430701845673No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16660.15343002679039303No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16410.15112765543999698No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCT16110.14836480981952171No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG15900.14643081788518902No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA15870.1461545333231415No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15850.14597034361510983No Hit
ATTTCTGGTGGCTCCGTCAGCACTAGTAATTATTACTGGGGCTGGATCCG15780.14532567963699894No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15780.14532567963699894No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTTATCTCTGAGGAT15470.14247073916250783No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA15440.1421944546004603No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC15390.14173398033038107No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15330.14118141120628602No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15260.14053674722817514No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14860.13685295306754144No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14820.13648457365147806No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14620.13464267657116125No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14600.13445848686312956No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14380.13243240007478102No Hit
CCTCTGGCACAGTAATATATGGCCGTGTCCGCGGCGGTCACAGACTTCAG14110.1299458390163533No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTTATCTCTGAGG14000.12893279562217902No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13880.12782765737398893No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGT13880.12782765737398893No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13570.12497271689949781No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13560.12488062204548196No Hit
GTGCCAGAGGCGCGTGGCAAGGGGTTTCGGGGTTCGCCCCCTGGGGCCAG13520.1245122426294186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13510.12442014777540276No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT13340.12285453525713343No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT13140.12101263817681658No Hit
CTCTGAGGATGACCAAAGACCCTCTGGAGTCCCTGATCGGTTCTCTGGCT12940.11917074109649975No Hit
CTCCTGCACCGGCAGCAGTGGCAGCATTGCCAACAACTTCGTGCAGTGGT12840.11824979255634133No Hit
ATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGA12780.11769722343224628No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12600.11603951605996111No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGACATTGGTGTTAT12490.11502647266578685No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12440.11456599839570765No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG12380.11401342927161259No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12350.11373714470956507No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12270.11300038587743833No Hit
ACCAAAGACCCTCTGGAGTCCCTGATCGGTTCTCTGGCTCCGTCGACATC12170.1120794373372799No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC12110.11152686821318486No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA12090.11134267850515317No Hit
GTCCAGAGATGGTGAGGGAGGCGGAATTGGAGGAGATGTCGACGGAGCCA11910.109684971132868No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG11900.10959287627885217No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11860.1092244968627888No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11580.10664584095034521No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG11510.10600117697223432No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11450.10544860784813927No Hit
CTGTAGCAGCCACAGAGGTCACGCTCAGGTGGTCCTTGAACAGCGCTCTT11210.10323833135175906No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG11180.10296204678971153No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT11150.10268576222766401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.088.1062851
GTATCAA19150.078.352481
TCAACGC24300.059.0016374
TATCAAC24950.058.3308262
ATCAACG24800.058.1026083
CAACGCA24900.057.2905655
AACGCAG25600.055.662456
GAATACG2300.053.1934479
ACGCAGA29150.048.8836567
CGCAGAG29250.048.7165348
GCAGAGT33700.042.4971929
GTGCTAG3900.038.843011
TATTGAG14500.035.7751775
TACATGG28450.035.200582
GTACATG28950.035.1340941
GTAATAT20750.034.417181
CAGAGTA32500.034.3230139
TGGCCGT20000.033.824779
ATGGCCG19900.033.63318
TTATTGA16100.031.7723794
Produced by FastQC (version 0.11.5)