FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085837
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTACTGTGCCAGAGGCGCGTGGCAAGGGGTTTCGGGGTTCGCCCC48720.44868612876518305No Hit
GTAATATATGGCCGTGTCCGCGGCGGTCACAGACTTCAGTTTCAGGGAGA40430.37233949478604983No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC28700.26431223102546697No Hit
GCCATATATTACTGTGCCAGAGGCGCGTGGCAAGGGGTTTCGGGGTTCGC27120.24976124409096392No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA23020.21200235394446865No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGACATTGGTGTTA21670.19956954865232995No Hit
GTCTTATGATAACACCAATGTCGTTTTCGGCGGAGGGACCAAGCTGACCG20550.18925492500255564No Hit
TCTCTGGACTAAGGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT20460.18842607131641306No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20050.18465018230176353No Hit
ATATATGGCCGTGTCCGCGGCGGTCACAGACTTCAGTTTCAGGGAGAACT19650.18096638814112984No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCCTTAGTCCAGA19080.17571698146222683No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA19040.17534860204616345No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18920.17424346379797337No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA18830.17341461011183076No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18520.17055966963733965No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAG18090.16659959091465845No Hit
ATATTACTGTGCCAGAGGCGCGTGGCAAGGGGTTTCGGGGTTCGCCCCCT17860.1644814092722941No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17750.16346836587811983No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17720.1631920813160723No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG17540.16153437394378714No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16900.15564030328677325No Hit
TCTTTAGACACTTCCAAGAATCAGTTCTCCCTGAAACTGAAGTCTGTGAC16790.15462725989259898No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGAC16780.15453516503858314No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16710.15389050106047225No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC16480.15177231941810787No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16080.14808852525747418No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG15960.1469833870092841No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA15720.14477311051290387No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15670.14431263624282464No Hit
GTCCAGAGATGGTGAGGGAGGCGGAATTGGAGGAGATGTCGACGGAGCCA15430.14210235974644445No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15140.13943160897998502No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCT15010.13823437587777906No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14640.1348268662791929No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14470.1332612537609236No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14430.13289287434486025No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14300.1316956412426543No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14180.13059050299446417No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC14090.1297616493083216No Hit
ATTTCTGGTGGCTCCGTCAGCACTAGTAATTATTACTGGGGCTGGATCCG14050.12939326989225824No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTTATCTCTGAGGAT14010.12902489047619486No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13680.1259857602936721No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13310.1225782506950859No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13230.12184149186295917No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTTATCTCTGAGG13140.12101263817681658No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12930.11907864624248392No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12900.11880236168043637No Hit
CCTCTGGCACAGTAATATATGGCCGTGTCCGCGGCGGTCACAGACTTCAG12840.11824979255634133No Hit
GTGCCAGAGGCGCGTGGCAAGGGGTTTCGGGGTTCGCCCCCTGGGGCCAG12570.1157632314979136No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12450.1146580932497235No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG12350.11373714470956507No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12320.11346086014751752No Hit
CTCTGAGGATGACCAAAGACCCTCTGGAGTCCCTGATCGGTTCTCTGGCT12290.11318457558547No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACGACATTGGTGTTAT12260.11290829102342248No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12160.11198734248326406No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12140.11180315277523238No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12070.1111584887971215No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11900.10959287627885217No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11850.10913240200877294No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11760.10830354832263038No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC11660.10738259978247196No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCCTTAGTCCAGAGATGGTGAGGGAGG11530.10618536668026601No Hit
CTCCTGCACCGGCAGCAGTGGCAGCATTGCCAACAACTTCGTGCAGTGGT11420.10517232328609175No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11350.10452765930798084No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11220.1033304262057749No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGT11200.10314623649774321No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11190.10305414164372738No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11110.10231738281160063No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG11080.10204109824955311No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA11020.10148852912545807No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10940.10075177029333132No Hit
GACTACTACTGTCAGTCTTATGATAACACCAATGTCGTTTTCGGCGGAGG10890.1002912960232521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.074.191531
GTATCAA14450.066.23841
ATCAACG14800.064.11173
TCAACGC15300.062.027994
CAACGCA15400.061.639435
TATCAAC15700.060.4365122
AACGCAG16450.057.7076576
GAATACG2650.051.56249
GTGCTAG3400.050.799381
CGCAGAG19250.049.3138168
ACGCAGA19300.049.1860587
GCAGAGT22550.042.097169
GTAATAT21450.035.228011
GCTAGAG4300.033.433873
TGGCCGT21200.032.5657279
TATTGAG15250.032.0659755
ATGGCCG22250.031.9985718
TACATGG25100.031.7888152
ATTGAGG7500.031.6430326
CTAGAGA5050.031.3210644
Produced by FastQC (version 0.11.5)