FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758867
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGGCCGTGTCCTCAAGTCTCAGACGCTTCATGTGCAGATACAGGG40800.537643618710525No Hit
GCCTTATATTACTGTGTTAAAGATATCGGTGACTCCTGGGGCCAGGGAAC37410.492971759214724No Hit
ACCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA23240.3062460220302108No Hit
TGGTAGCACTATCCCAACTAATACTTGAGACCCACTCCAGGCCCTTCCCT22000.2899058728341066No Hit
GTAATAATCAGCCTCATCATCAGACTGGAGCCCACTGATGGCCAGGGAGG21010.27686010855657184No Hit
GTGCTACCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCC20190.26605452602366425No Hit
GCCTATGGTAGCACTATCCCAACTAATACTTGAGACCCACTCCAGGCCCT16850.22204154351157715No Hit
GATTATTACTGTGCGGCATGGGATGAGAGCCTGAATGGTTATGCCTTCGG16480.2171658538320944No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15630.20596494510895846No Hit
ATATTACTGTGTTAAAGATATCGGTGACTCCTGGGGCCAGGGAACCCTGG14470.1906789990867965No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13820.18211359829851606No Hit
CAGTAATATAAGGCCGTGTCCTCAAGTCTCAGACGCTTCATGTGCAGATA13310.1753930530646345No Hit
CTTTAACACAGTAATATAAGGCCGTGTCCTCAAGTCTCAGACGCTTCATG12750.16801363084703907No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12340.16261083958058528No Hit
GTGTTAAAGATATCGGTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTC12150.16010710704247252No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12130.159843556248987No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12000.15813047609133088No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC11720.15444076498253317No Hit
GTAATATAAGGCCGTGTCCTCAAGTCTCAGACGCTTCATGTGCAGATACA11420.15048750308024988No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11420.15048750308024988No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG10860.14310808086265445No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10830.14271275467242614No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT10580.13941836975385674No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGACTTGGGACTGAGCTGGA10540.13889126816688563No Hit
GCACTGAACACAGAGGACTCACCATGGACTTGGGACTGAGCTGGATTTTC10480.13810061578642896No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10260.13520155705808792No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10150.1337520276939174No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT10030.13217072293300408No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTGATGATAATGCCATGCACTGGG9850.1297987657916341No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC9650.1271632578567786No Hit
GAACACAGAGGACTCACCATGGACTTGGGACTGAGCTGGATTTTCCTTTT9580.12624083007957917No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9540.12571372849260806No Hit
CTCCAGGCCCTTCCCTGGAACTTGGCGGACCCAGTGCATGGCATTATCAT9530.12558195309586528No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA9220.12149691579683923No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9090.11978383563918316No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9050.11925673405221206No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCATAACCATTCAGG8970.11820253087826983No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCATAACCATTCAGGC8870.11688477691084208No Hit
TTATATTACTGTGTTAAAGATATCGGTGACTCCTGGGGCCAGGGAACCCT8800.11596234913364264No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8730.11503992135644323No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA8710.11477637056295767No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT8690.11451281976947213No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT8670.11424926897598657No Hit
GACTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCA8500.11200908723135938No Hit
CTCCAGAGACAACGCCAAGAACTCCCTGTATCTGCACATGAAGCGTCTGA8480.11174553643787384No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC8430.11108665945415996No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8380.11042778247044609No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8170.10766049913884779No Hit
GCCATGCACTGGGTCCGCCAAGTTCCAGGGAAGGGCCTGGAGTGGGTCTC8020.10568386818770614No Hit
GTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAAACTTGGTGCAGCCTGGCA7950.10476144041050672No Hit
GGCCTGGAGTGGGTCTCAAGTATTAGTTGGGATAGTGCTACCATAGGCTA7930.10449788961702117No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG7890.10397078803005007No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATCTTTGATGATAATGCC7840.10331191104633619No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAAACT7730.10186238168216565No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7730.10186238168216565No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7620.10041285231799511No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC7590.10001752612776679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14850.059.305881
ATCAACG15800.056.9563943
TCAACGC16000.056.244444
TATCAAC16600.053.3968962
CAACGCA17100.052.626385
ACACCGG701.7675024E-551.423496
GGTATCA6900.051.2638661
AACGCAG17850.050.4151846
ACGCAGA19650.045.7977
CGCAGAG20150.044.66068
GCAGAGT21800.041.6105659
GTGCTAG3450.035.5708471
GAATACG2404.7293724E-1132.996749
TACATGG20350.032.2252272
GTACATG20650.032.1611941
GCATCGC900.00375453531.9968389
GTACTAG1153.2423786E-431.3860421
TACACCG950.004891666630.3127945
CTAACAC2452.1245796E-929.3848483
AGGGGTG6650.029.2301925
Produced by FastQC (version 0.11.5)