FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758867
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGGCCGTGTCCTCAAGTCTCAGACGCTTCATGTGCAGATACAGGG34910.4600279100290301No Hit
GCCTTATATTACTGTGTTAAAGATATCGGTGACTCCTGGGGCCAGGGAAC34710.4573924020941746No Hit
ACCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA18760.24721064428944728No Hit
GTGCTACCATAGGCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCC18510.2439162593708779No Hit
TGGTAGCACTATCCCAACTAATACTTGAGACCCACTCCAGGCCCTTCCCT18440.24299383159367846No Hit
GCCTATGGTAGCACTATCCCAACTAATACTTGAGACCCACTCCAGGCCCT15640.20609672050570124No Hit
GTAATAATCAGCCTCATCATCAGACTGGAGCCCACTGATGGCCAGGGAGG13790.18171827210828775No Hit
GATTATTACTGTGCGGCATGGGATGAGAGCCTGAATGGTTATGCCTTCGG13480.17763323480926171No Hit
ATATTACTGTGTTAAAGATATCGGTGACTCCTGGGGCCAGGGAACCCTGG12510.16485102132521245No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12000.15813047609133088No Hit
CAGTAATATAAGGCCGTGTCCTCAAGTCTCAGACGCTTCATGTGCAGATA11790.1553631927597326No Hit
CTTTAACACAGTAATATAAGGCCGTGTCCTCAAGTCTCAGACGCTTCATG10750.14165855149848391No Hit
GTGTTAAAGATATCGGTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTC10610.13981369594408508No Hit
GTAATATAAGGCCGTGTCCTCAAGTCTCAGACGCTTCATGTGCAGATACA10600.1396819205473423No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10560.13915481896037118No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9950.13111651975906186No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT9880.13019409198186244No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC9310.12268289436752422No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9210.12136514040009647No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGACTTGGGACTGAGCTGGA8950.1179389800847843No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC8880.11701655230758486No Hit
CTCCAGGCCCTTCCCTGGAACTTGGCGGACCCAGTGCATGGCATTATCAT8490.11187731183461659No Hit
GCACTGAACACAGAGGACTCACCATGGACTTGGGACTGAGCTGGATTTTC8390.11055955786718884No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTGATGATAATGCCATGCACTGGG8350.11003245628021774No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8260.10884647770953276No Hit
TTATATTACTGTGTTAAAGATATCGGTGACTCCTGGGGCCAGGGAACCCT8230.10845115151930443No Hit
GAACACAGAGGACTCACCATGGACTTGGGACTGAGCTGGATTTTCCTTTT8160.10752872374210501No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT8160.10752872374210501No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8140.10726517294861945No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA7960.1048932158072495No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7830.10318013564959341No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7760.10225770787239397No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7750.10212593247565122No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7720.10173060628542287No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT7640.10067640311148067No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7620.10041285231799511No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)