FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817700
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCGTCAGATCTCAGACTGCTCAGTTCCATGTAGA47380.5794301088418736No Hit
GTCTACATGGAACTGAGCAGTCTGAGATCTGACGACACGGCCATGTATTA34540.42240430475724594No Hit
CAATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG29680.3629693041457747No Hit
CATGTAGACTGTGCTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCT27710.3388773388773389No Hit
CCCTTATAGTGGTGGCACAAACTATGCACAGAACTTTCAGGGCAGGGTCA27600.3375321022379846No Hit
TCTGGATACAGTGTCACCGACCACAATATGCACTGGGTGCGACAGGCCCC27370.3347193347193347No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCA26230.32077779136602663No Hit
ATGTATTACTGTGCGAGAGGGGGTGGGAGGTATCACCTTGACTACTGGGG25020.3059801883331295No Hit
CTATAAGGGATGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG23970.2931392931392931No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT22100.2702702702702703No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA20090.2456891280420692No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC19650.24030818148465205No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT18010.22025192613427907No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT18010.22025192613427907No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTG17910.21902898373486607No Hit
CAGTAATACATGGCCGTGTCGTCAGATCTCAGACTGCTCAGTTCCATGTA16940.2071664424605601No Hit
GTCTGAGATCTGACGACACGGCCATGTATTACTGTGCGAGAGGGGGTGGG16550.20239696710284946No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT15970.19530390118625413No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG15700.19200195670783907No Hit
ATCTCAGACTGCTCAGTTCCATGTAGACTGTGCTGATGGACGTGTCCCTG15480.18931148342913048No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15290.1869878928702458No Hit
ATACATGGCCGTGTCGTCAGATCTCAGACTGCTCAGTTCCATGTAGACTG15260.18662101015042193No Hit
GTATTACTGTGCGAGAGGGGGTGGGAGGTATCACCTTGACTACTGGGGCC15190.1857649504708328No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT14720.18001712119359178No Hit
GACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATTATTGCTG14570.1781827075944723No Hit
ACATGGAACTGAGCAGTCTGAGATCTGACGACACGGCCATGTATTACTGT14550.1779381191145897No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCATC14530.1776935306347071No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14200.17365782071664426No Hit
ACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATTATTGCTGC13980.17096734743793565No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT13210.16155069096245567No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA12990.1588602176837471No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC12860.15727039256451023No Hit
GTAATAATCAGCTTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG12750.15592515592515593No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12650.15470221352574293No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12500.15286779992662347No Hit
GTTCCATGTAGACTGTGCTGATGGACGTGTCCCTGGTCATGGTGACCCTG12460.15237862296685825No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACC12320.15066650360768008No Hit
GTGCTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCTGAAAGTTCTG12210.1493212669683258No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG12060.1474868533692063No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11900.14553014553014554No Hit
CTCCTGGACAGTCAGTCATCGTCTCCTGCACTGGAACCAGCAGTGACGTT11560.14137214137214135No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT11360.1389262565733154No Hit
GATTATTACTGCATGTCACATGCAGGCAGCAATAATTATGTCTTCGGAAC11340.1386816680934328No Hit
TCTCTGGGCTCCAGGCTGAGGATGAAGCTGATTATTACTGCATGTCACAT11340.1386816680934328No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11320.1384370796135502No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA11020.13476825241531123No Hit
GACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCCACAGGAGCCCACTCCC10920.13354531001589826No Hit
GCCATGTATTACTGTGCGAGAGGGGGTGGGAGGTATCACCTTGACTACTG10820.13232236761648528No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT10710.13097713097713096No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG10680.13061024825730708No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC10530.1287758346581876No Hit
GGATGGATCATCCCTTATAGTGGTGGCACAAACTATGCACAGAACTTTCA10460.12791977497859852No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10420.12743059801883333No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA10390.12706371529900942No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG10110.12363947658065304No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG10080.12327259386082916No Hit
CATCAGCACAGTCTACATGGAACTGAGCAGTCTGAGATCTGACGACACGG10080.12327259386082916No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT10030.12266112266112265No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9900.12107129754188578No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9750.11923688394276628No Hit
GTGCATATTGTGGTCGGTGACACTGTATCCAGAAGCCTTGCAGGAGACCC9740.11911458970282499No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC9590.11728017610370552No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9490.11605723370429254No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT9260.11324446618564264No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA9130.11165464106640578No Hit
CAGTGTCACCGACCACAATATGCACTGGGTGCGACAGGCCCCTGGACAAG9130.11165464106640578No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC9110.11141005258652319No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG9080.11104316986669928No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9000.1100648159471689No Hit
GTACATGGGGAGCATCACCCAGCAACCACATCTGTCCTCTAGAGAATCCC8990.1099425217072276No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG8950.10945334474746239No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8920.1090864620276385No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC8760.10712975418857773No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG8740.10688516570869512No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC8630.10553992906934084No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCATCCCTTATAGTGGT8610.10529534058945825No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA8500.10395010395010396No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA8500.10395010395010396No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8350.10211569035098447No Hit
GCTCAGTTCCATGTAGACTGTGCTGATGGACGTGTCCCTGGTCATGGTGA8350.10211569035098447No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG8310.10162651339121927No Hit
CCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCCACAGGA8210.10040357099180629No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8200.10028127675186499No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)