FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817700
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCGTCAGATCTCAGACTGCTCAGTTCCATGTAGA44220.5407851290204231No Hit
GTCTACATGGAACTGAGCAGTCTGAGATCTGACGACACGGCCATGTATTA30710.3755656108597285No Hit
CATGTAGACTGTGCTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCT25970.3175981411275529No Hit
CTATAAGGGATGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG24790.3031674208144796No Hit
CAATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG23980.29326158737923447No Hit
CCCTTATAGTGGTGGCACAAACTATGCACAGAACTTTCAGGGCAGGGTCA23260.28445640210346096No Hit
TCTGGATACAGTGTCACCGACCACAATATGCACTGGGTGCGACAGGCCCC22320.27296074354897887No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCA22000.2690473278708573No Hit
ATGTATTACTGTGCGAGAGGGGGTGGGAGGTATCACCTTGACTACTGGGG21270.2601198483551425No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT17890.2187843952549835No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA17820.21792833557539437No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT16150.19750519750519752No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT16070.1965268435856671No Hit
CAGTAATACATGGCCGTGTCGTCAGATCTCAGACTGCTCAGTTCCATGTA15880.19420325302678246No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC15690.19187966246789775No Hit
ATCTCAGACTGCTCAGTTCCATGTAGACTGTGCTGATGGACGTGTCCCTG14730.18013941543353307No Hit
ATACATGGCCGTGTCGTCAGATCTCAGACTGCTCAGTTCCATGTAGACTG14440.1765928824752354No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13940.1704781704781705No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG13620.1665647548000489No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13590.16619787208022502No Hit
GTATTACTGTGCGAGAGGGGGTGGGAGGTATCACCTTGACTACTGGGGCC13220.16167298520239698No Hit
GTCTGAGATCTGACGACACGGCCATGTATTACTGTGCGAGAGGGGGTGGG13220.16167298520239698No Hit
GTGCTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCTGAAAGTTCTG12600.15409074232603645No Hit
GACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATTATTGCTG12300.15042191512779748No Hit
ACCTAGGACGGTGACTTTGGTCCCAGTTCCGAAGACATAATTATTGCTGC12060.1474868533692063No Hit
ACATGGAACTGAGCAGTCTGAGATCTGACGACACGGCCATGTATTACTGT12050.147364559129265No Hit
GTTCCATGTAGACTGTGCTGATGGACGTGTCCCTGGTCATGGTGACCCTG12020.1469976764094411No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCATC12010.14687538216949983No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTG11960.14626391096979333No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11730.14345114345114346No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11420.1396600220129632No Hit
GTGCATATTGTGGTCGGTGACACTGTATCCAGAAGCCTTGCAGGAGACCC11170.13660266601443072No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC11120.13599119481472421No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT11060.13525742937507645No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA10990.13440136969548733No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10820.13232236761648528No Hit
GTAATAATCAGCTTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG10630.12999877705760057No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA10450.1277974807386572No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10450.1277974807386572No Hit
GATTATTACTGCATGTCACATGCAGGCAGCAATAATTATGTCTTCGGAAC10140.12400635930047695No Hit
CTCCTGGACAGTCAGTCATCGTCTCCTGCACTGGAACCAGCAGTGACGTT10130.12388406506053565No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10130.12388406506053565No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG10000.12229423994129876No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9780.11960376666259019No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9570.11703558762382292No Hit
TCTCTGGGCTCCAGGCTGAGGATGAAGCTGATTATTACTGCATGTCACAT9350.11434511434511435No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT9310.11385593738534915No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACC9290.11361134890546655No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA9250.11312217194570137No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9080.11104316986669928No Hit
GGATGGATCATCCCTTATAGTGGTGGCACAAACTATGCACAGAACTTTCA9080.11104316986669928No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT9070.11092087562675798No Hit
GCCATGTATTACTGTGCGAGAGGGGGTGGGAGGTATCACCTTGACTACTG9030.11043169866699278No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT8860.10835269658799071No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG8690.10627369450898862No Hit
CATCAGCACAGTCTACATGGAACTGAGCAGTCTGAGATCTGACGACACGG8640.10566222330928214No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG8600.10517304634951694No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8480.10370551547022136No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8370.10236027883086707No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA8320.10174880763116056No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC8300.10150421915127797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11050.056.0776631
TCAACGC11200.054.6331334
ATCAACG11450.052.1828543
CAACGCA11900.052.0243575
TATCAAC12200.051.3351252
AACGCAG12700.049.314066
GAATACG1653.092282E-1143.62869
CGCAGAG14400.040.9927028
GTGCTAG1807.6397555E-1140.02961
GTATGTA9000.038.4284131
ATTGCCG750.001535875138.393178
GTACATG21300.035.8575021
TATGTAC8550.035.362132
GCAGAGT16300.035.3311359
CATGGGG16700.034.484884
GTACACG8200.034.2378125
TACACGG8300.032.957996
ACATGGG23000.032.8637163
GAGTTAA900.003739319532.0236781
ATGGGGC4050.031.9943085
Produced by FastQC (version 0.11.5)