FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985031
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.15878845217026838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41850.068.645141
TCAACGC45250.061.741664
ATCAACG46500.060.081943
CAACGCA48550.057.5450065
GGTATCA22250.055.3348161
AACGCAG51150.055.043656
TATCAAC54600.051.6961982
ACGCAGA56800.049.4415867
CGCAGAG63900.043.8353848
GCAGAGT73700.038.0007869
GTACATG60200.029.063111
TACATGG61350.027.3468022
ACATGGG59200.026.637143
CAGAGTA68600.022.8793059
TATAAGG8450.018.7469732
CATGGGA28700.018.5658574
CATGGGG44650.018.5456284
TATACTG13750.017.8049765
AGAGTAC63450.017.58811610-14
CCTATAC9850.017.5444683
Produced by FastQC (version 0.11.5)